Dynamic Sorting of Mobile and Rigid Molecules in Biomembranes by Magic-Angle Spinning 13 C NMR

• Published in: Analytical chemistry (Washington) 2023-02, Vol.95 (7), p.3596-3605
• Main Authors: Morvan, Estelle, Taib-Maamar, Nada, Grélard, Axelle, Loquet, Antoine, Dufourc, Erick J
• Format: Article
• Language: English
• Subjects: Biochemistry, Molecular Biology; Chemical Sciences; Life Sciences; or physical chemistry; Structural Biology; Theoretical and
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.2c04185

Understanding the membrane dynamics of complex systems is essential to follow their function. As molecules in membranes can be in a rigid or mobile state depending on external (temperature, pressure) or internal (pH, domains, etc.) conditions, we propose an in-depth examination of NMR methods to filter highly mobile molecular parts from others that are in more restricted environments. We have thus developed a quantitative magic-angle spinning (MAS) C NMR approach coupled with cross-polarization (CP) and/or Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) on rigid and fluid unlabeled model membranes. We demonstrate that INEPT can detect only very mobile lipid headgroups in gel (solid-ordered) phases; the remaining rigid parts are only detected with CP. A direct correlation is established between the normalized line intensity as obtained by CP and the C-H (C-D) order parameters measured by wide-line H NMR or extracted from molecular dynamics: / ≈ 5| |, indicating that when the order is greater than 0.2-0.3 (maximum value of 0.5 for chain CH ), only rigid parts can be filtered and detected using CP techniques. In very fluid (liquid-disordered) membranes, where there are many more active motions, both INEPT and CP detect resonances, with, however, a clear propensity of each technique to detect mobile and restricted molecular parts, respectively. Interestingly, the C NMR chemical shift of lipid hydrocarbon chains can be used to monitor order-disorder phase transitions and calculate the fraction of chain defects (rotamers) and the part of the transition enthalpy due to bond rotations (6-7 kJ·mol for dimyristolphosphatidylcholine, DMPC). Cholesterol-containing membranes (liquid-ordered phases) can be dynamically contrasted as the rigid-body sterol is mainly detected by the CP technique, with a contact time of 1 ms, and the phospholipid by INEPT. Our work opens up a straightforward, robust, and cost-effective route for the determination of membrane dynamics by taking advantage of well-resolved conventional C NMR experiments without the need of isotopic labeling.