A physical map of human chromosome 14

We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogene...

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Bibliographic Details
Published in:Nature (London) 2001-02, Vol.409 (6822), p.947-948
Main Authors: Heilig, Roland, Brüls, Thomas, Gyapay, Gabor, Petit, Jean-Louis, Artiguenave, François, Vico, Virginie, Qin, Shizen, Tin-Wollam, Aye Mon, Da Silva, Corinne, Muselet, Delphine, Mavel, Delphine, Pelletier, Eric, Levy, Michael, Fujiyama, Asao, Matsuda, Fumihiko, Wilson, Richard, Rowen, Lee, Hood, Leroy, Weissenbach, Jean, Saurin, William
Format: Article
Language:English
Subjects:
Bacteria
chromosome 14
Chromosomes
Chromosomes, Artificial, Bacterial
Chromosomes, Human, Pair 14
Cloning
Cloning, Molecular
Connectors
Continuity
Deoxyribonucleic acid
DNA
Escherichia coli
Genes
Genomics
Human
Humanities and Social Sciences
Humans
letters-to-nature
Life Sciences
multidisciplinary
Physical Chromosome Mapping
Radiation Hybrid Mapping
Science
Seeds
Sequence Tagged Sites
Strategy
Tiling
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Summary:We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.
ISSN:0028-0836
1476-4687
DOI:10.1038/35057177