Direct modulation of volume-regulated anion channels by Ca 2+ chelating agents

Ca 2+ chelating agents are widely used in biological research for Ca 2+ buffering. Here we report that BAPTA, EDTA and HEDTA produce fast, reversible, voltage-dependent inhibition of swelling-activated Cl − current ( I Cl,swell) in LNCaP prostate cancer epithelial cells that is unrelated to their Ca...

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Bibliographic Details
Published in:FEBS letters 2002-06, Vol.521 (1), p.152-156
Main Authors: Lemonnier, L, Vitko, Y, Shuba, Y.M, Vanden Abeele, F, Prevarskaya, N, Skryma, R
Format: Article
Language:English
Subjects:
BAPTA
Calcium chelating agent
EDTA
Life Sciences
LNCaP prostate cancer cell
Swelling-activated chloride current
Volume-regulated anion channel
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Summary:Ca 2+ chelating agents are widely used in biological research for Ca 2+ buffering. Here we report that BAPTA, EDTA and HEDTA produce fast, reversible, voltage-dependent inhibition of swelling-activated Cl − current ( I Cl,swell) in LNCaP prostate cancer epithelial cells that is unrelated to their Ca 2+ binding. BAPTA was the most effective (maximal blockade 67%, IC 50=70 μM, at +100 mV) followed by EDTA and HEDTA. I Cl,swell blockade by EDTA was pH-dependent. BAPTA blocked I Cl,swell also in other cell types. We conclude that Ca 2+ chelating agents block I Cl,swell by acting directly on the underlying channel, and that the negative charge of the free chelator form is critical for the blockade.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)02863-6