Calcareous impact on arbuscular mycorrhizal fungus development and on lipid peroxidation in monoxenic roots

• Published in: Phytochemistry (Oxford) 2011-12, Vol.72 (18), p.2335-2341
• Main Authors: Labidi, Sonia, Calonne, Maryline, Ben Jeddi, Fayçal, Debiane, Djouher, Rezgui, Salah, Laruelle, Frédéric, Tisserant, Benoit, Grandmougin-Ferjani, Anne, Lounès-Hadj Sahraoui, Anissa
• Format: Article
• Language: English
• Subjects: Antioxidants; Arbuscular mycorrhiza; arbuscular mycorrhizas; Biological and medical sciences; CaCO 3; Calcium Carbonate - pharmacology; Cell membranes; Chemical constitution; chicory; Cichorium intybus - drug effects; Cichorium intybus - metabolism; Cichorium intybus - microbiology; Colonization; Development; Elongation; Enzymes; Fatty acids; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Glomeromycota - drug effects; Glomeromycota - growth & development; Glomeromycota - metabolism; Glomus; Glomus irregulare; Hyphae; Life cycle; Life Sciences; Lipid Peroxidation; Malondialdehyde; Mycorrhizae - drug effects; Mycorrhizae - growth & development; Mycorrhizae - metabolism; mycorrhizal fungi; Peroxidase; Plant physiology and development; Plant Roots - drug effects; Plant Roots - metabolism; Plant Roots - microbiology; root growth; Roots; Spore germination; Sporulation; Stress, Physiological; Symbiosis; vesicular arbuscular mycorrhizae
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2011.08.016

In the presence of increasing CaCO 3 concentrations, malondialdehyde (MDA) formation was found to be increased only in the non-mycorrhizal (NM) chicory roots and not in the mycorrhizal (M) ones. This result suggests the involvement of AMF in the chicory root protection against carbonates stress by limiting lipid peroxidation. [Display omitted] ► The study pointed out, for the first time, the CaCO 3 negative effects on the arbuscular mycorrhizal symbiosis. ► CaCO 3 impaired the main stages of Glomus irregulare life cycle but did not inhibit it completely. ► Root colonization reduction was confirmed by the AM fungal marker C16:1ω5 decrease. ► Oxidative stress was evaluated by fatty acid analysis, malondialdehyde formation and peroxidase assessment. ► AM symbiosis enhance the plant tolerance to CaCO 3 by preventing lipid peroxidation. The present work underlined the negative effects of increasing CaCO 3 concentrations (5, 10 and 20 mM) both on the chicory root growth and the arbuscular mycorrhizal fungus (AMF) Glomus irregulare development in monoxenic system. CaCO 3 was found to reduce drastically the main stages of G. irregulare life cycle (spore germination, germinative hyphae elongation, root colonization, extraradical hyphae development and sporulation) but not to inhibit it completely. The root colonization drop was confirmed by the decrease in the arbuscular mycorrhizal fungal marker C16:1ω5 amounts in the mycorrhizal chicory roots grown in the presence of CaCO 3. Oxidative damage evaluated by lipid peroxidation increase measured by (i) malondialdehyde (MDA) production and (ii) the antioxidant enzyme peroxidase (POD) activities, was highlighted in chicory roots grown in the presence of CaCO 3. However, MDA formation was significantly higher in non-mycorrhizal roots as compared to mycorrhizal ones. This study pointed out the ability of arbuscular mycorrhizal symbiosis to enhance plant tolerance to high levels of CaCO 3 by preventing lipid peroxidation and so less cell membrane damage.