Microbial predators form a new supergroup of eukaryotes

• Published in: Nature (London) 2022-12, Vol.612 (7941), p.714-719
• Main Authors: Tikhonenkov, Denis V., Mikhailov, Kirill V., Gawryluk, Ryan M. R., Belyaev, Artem O., Mathur, Varsha, Karpov, Sergey A., Zagumyonnyi, Dmitry G., Borodina, Anastasia S., Prokina, Kristina I., Mylnikov, Alexander P., Aleoshin, Vladimir V., Keeling, Patrick J.
• Format: Article
• Language: English
• Subjects: 14/28; 14/63; 45/22; 45/23; 45/29; 45/77; 45/90; 45/91; 631/158/855; 631/181/2480; 631/181/735; 631/337/2019; Aquatic Organisms - classification; Aquatic Organisms - genetics; Aquatic Organisms - ultrastructure; Biodiversity; Divergence; Ecology; Eukaryota - classification; Eukaryota - genetics; Eukaryota - ultrastructure; Eukaryotes; Eukaryotic Cells - classification; Eukaryotic Cells - metabolism; Eukaryotic Cells - ultrastructure; Food Chain; Freshwater environments; Humanities and Social Sciences; Life Sciences; Microbiology; Microorganisms; Morphology; multidisciplinary; Phylogenetics; Phylogeny; Predators; Predatory Behavior; Science; Science (multidisciplinary); Species Specificity; Taxonomy; Ultrastructure
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/s41586-022-05511-5

Molecular phylogenetics of microbial eukaryotes has reshaped the tree of life by establishing broad taxonomic divisions, termed supergroups, that supersede the traditional kingdoms of animals, fungi and plants, and encompass a much greater breadth of eukaryotic diversity 1 . The vast majority of newly discovered species fall into a small number of known supergroups. Recently, however, a handful of species with no clear relationship to other supergroups have been described 2 – 4 , raising questions about the nature and degree of undiscovered diversity, and exposing the limitations of strictly molecular-based exploration. Here we report ten previously undescribed strains of microbial predators isolated through culture that collectively form a diverse new supergroup of eukaryotes, termed Provora. The Provora supergroup is genetically, morphologically and behaviourally distinct from other eukaryotes, and comprises two divergent clades of predators—Nebulidia and Nibbleridia—that are superficially similar to each other, but differ fundamentally in ultrastructure, behaviour and gene content. These predators are globally distributed in marine and freshwater environments, but are numerically rare and have consequently been overlooked by molecular-diversity surveys. In the age of high-throughput analyses, investigation of eukaryotic diversity through culture remains indispensable for the discovery of rare but ecologically and evolutionarily important eukaryotes. Provora is an ancient supergroup of microbial predators that are genetically, morphologically and behaviourally distinct from other eukaryotes, and comprise two divergent clades of predators—Nebulidia and Nibbleridia—that differ fundamentally in ultrastructure, behaviour and gene content.