Correlation between plant cell wall stiffening and root extension arrest phenotype in the combined abiotic stress of Fe and Al

• Published in: Plant, cell and environment cell and environment, 2024-02, Vol.47 (2), p.574-584
• Main Authors: Kaur, Harinderbir, Teulon, Jean‐Marie, Godon, Christian, Desnos, Thierry, Chen, Shu‐wen W., Pellequer, Jean‐Luc
• Format: Article
• Language: English
• Subjects: Aluminum; Aluminum - pharmacology; Arabidopsis; Arabidopsis Proteins - genetics; Atomic force microscopy; atomic force microscopy (AFM); Biomechanics; Botanics; Cell Wall; Cell walls; Development Biology; elasticity; Exudation; Ferrous ions; Ions; Iron; Life Sciences; Malate; Malates; Mechanics; Metal ions; Mutants; nanoindentation; Phenotypes; Physics; Plant growth; Plant Roots; primary root; Seedlings; Stiffening; Stiffness; Transition zone; trimechanic‐3PCS; Vegetal Biology
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.14744

The plasticity and growth of plant cell walls (CWs) remain poorly understood at the molecular level. In this work, we used atomic force microscopy (AFM) to observe elastic responses of the root transition zone of 4‐day‐old Arabidopsis thaliana wild‐type and almt1‐mutant seedlings grown under Fe or Al stresses. Elastic parameters were deduced from force‐distance curve measurements using the trimechanic‐3PCS framework. The presence of single metal species Fe2+ or Al3+ at 10 µM exerts no noticeable effect on the root growth compared with the control conditions. On the contrary, a mix of both the metal ions produced a strong root‐extension arrest concomitant with significant increase of CW stiffness. Raising the concentration of either Fe2+ or Al3+ to 20 µM, no root‐extension arrest was observed; nevertheless, an increase in root stiffness occurred. In the presence of both the metal ions at 10 µM, root‐extension arrest was not observed in the almt1 mutant, which substantially abolishes the ability to exude malate. Our results indicate that the combination of Fe2+ and Al3+ with exuded malate is crucial for both CW stiffening and root‐extension arrest. However, stiffness increase induced by single Fe2+ or Al3+ is not sufficient for arresting root growth in our experimental conditions. Summary statement We investigate the change in stiffness of the external primary cell wall of living Arabidopsis thaliana seedlings in the presence of metallic stress using atomic force microscopy. Results reveal for the first time the uncoupling between mechanical response (CW stiffening) and root extension arrest.