A Novel Missense Single Nucleotide Polymorphism in the GREM1 Gene is Highly Associated with Higher Reproductive Traits in Awassi Sheep

GREM1 (gremlin1) is a known inhibitor for BMP15 (bone morphogenetic protein 15) family, but its genetic diversity in sheep is unknown. The present study was conducted to analyze the polymorphism of GREM1 gene using PCR- single-strand conformation polymorphism (SSCP) and DNA sequencing methods and to...

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Bibliographic Details
Published in:Biochemical genetics 2021-04, Vol.59 (2), p.422-436
Main Authors: Imran, Faris S., Al-Thuwaini, Tahreer M., Al-Shuhaib, Mohammed Baqur S., Lepretre, Frederic
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Deoxyribonucleic acid
DNA
DNA sequencing
Fecundity
Gene polymorphism
Genetic diversity
Human Genetics
Inhibitors
Life Sciences
Litter size
Marker-assisted selection
Markers
Medical Microbiology
Nucleotides
Original Article
Polymorphism
Reproduction
Sheep
Single-nucleotide polymorphism
Statistical analysis
Structural stability
Structure-function relationships
Zoology
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Summary:GREM1 (gremlin1) is a known inhibitor for BMP15 (bone morphogenetic protein 15) family, but its genetic diversity in sheep is unknown. The present study was conducted to analyze the polymorphism of GREM1 gene using PCR- single-strand conformation polymorphism (SSCP) and DNA sequencing methods and to assess the possible association of GREM1 gene polymorphism with reproductive traits in Awassi ewes. A total of 224 ewes, 124 producing singles and 100 producing twins, were included in the study. Two SSCP patterns were detected in two amplified loci within the exon 2. Two exonic novel single nucleotide polymorphism (SNP)s were identified, c.74 T > G (the silent SNP p.Met123 =) and c.30 T > A with (the missense SNP p.Ile237Phe). Statistical analyses indicated a non-significant ( P  > 0.05) association of p.Met123 = with the analyzed reproductive traits of fecundity, prolificacy, litter size, and twinning rate. Meanwhile, p.Ile237Phe SNP exhibited a highly significant ( P  
ISSN:0006-2928
1573-4927
1573-4927
DOI:10.1007/s10528-020-10006-x