Food peptidomics of in vitro gastrointestinal digestions of partially purified bovine hemoglobin: low-resolution versus high-resolution LC-MS/MS analyses

Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health‐promoting effects. In the meat production food chain,...

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Bibliographic Details
Published in:Electrophoresis 2016-07, Vol.37 (13), p.1814-1822
Main Authors: Caron, Juliette, Chataigné, Gabrielle, Gimeno, Jean-Pascal, Duhal, Nathalie, Goossens, Jean-François, Dhulster, Pascal, Cudennec, Benoit, Ravallec, Rozenn, Flahaut, Christophe
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Cattle
Chromatography, Liquid - methods
Digestion
Food Analysis
Foodomics
Foods
Gastrointestinal digestion
Health
Hemoglobin
Hemoglobins - metabolism
In Vitro Techniques
In vitro testing
Life Sciences
NanoLC-HR-ESI-MS/MS
Peptide Mapping
Peptides
Peptides - metabolism
Peptidomics
Proteins
Proteomics
Tandem Mass Spectrometry - methods
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Summary:Consumers and governments have become aware how the daily diet may affect the human health. All proteins from both plant and animal origins are potential sources of a wide range of bioactive peptides and the large majority of those display health‐promoting effects. In the meat production food chain, the slaughterhouse blood is an inevitable co‐product and, today, the blood proteins remain underexploited despite their bioactive potentiality. Through a comparative food peptidomics approach we illustrate the impact of resolving power, accuracy, sensitivity, and acquisition speed of low‐resolution (LR)‐ and high‐resolution (HR)‐LC‐ESI‐MS/MS on the obtained peptide mappings and discuss the limitations of MS‐based peptidomics. From in vitro gastrointestinal digestions of partially purified bovine hemoglobin, we have established the peptide maps of each hemoglobin chain. LR technique (normal bore C18 LC‐LR‐ESI‐MS/MS) allows us to identify without ambiguity 75 unique peptides while the HR approach (nano bore C18 LC‐HR‐ESI‐MS/MS) unambiguously identify more than 950 unique peptides (post‐translational modifications included). Herein, the food peptidomics approach using the most performant separation methods and mass spectrometers with high‐resolution capabilities appears as a promising source of information to assess the health potentiality of proteins.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201500559