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Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment
Purpose Helcococcus kunzii is a skin commensal, Gram‐positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co‐isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting...
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| Published in: | Proteomics. Clinical applications 2023-07, Vol.17 (4), p.e2200069-n/a |
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| container_title | Proteomics. Clinical applications |
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| creator | Durand, Benjamin A. R. N. Dunyach‐Remy, Catherine El Kaddouri, Oumayma Daher, Riham Lavigne, Jean‐Philippe Armengaud, Jean Grenga, Lucia |
| description | Purpose
Helcococcus kunzii is a skin commensal, Gram‐positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co‐isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.
Experimental Design
The H. kunzii cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q‐Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.
Results
The H. kunzii’s cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative H. kunzii targets involved in modulating the virulence of S. aureus is investigated.
Conclusion and Clinical Relevance
This work, pioneering the study of H. kunzii physiology in a chronic wound‐like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management. |
| doi_str_mv | 10.1002/prca.202200069 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_04459508v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2841733185</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3978-c954f3a646e5f703afba8dbac1d737b2b1838339c1e9f516bf5b5a7f8fed8e913</originalsourceid><addsrcrecordid>eNqF0c1OGzEQB3ALFTVAe-VYrdQLHBL8ues9RhE0SJGIUNur5fWOi5PddWrvguDUR-AZeRIcBXLgUvng0ejn0Vh_hE4JnhCM6cUmGD2hmFKMcV4eoCMiczqWTPBP-5rnI3Qc4wpjwWmBP6MRK3BOpWRH6Pcy-B5860zmuuj-3PUxFb3P5tAYn44ZYrYeuifnUj_TWe10BX3i1vs-GxoD4eXfc-PWkEF374LvWuj6L-jQ6ibC17f7BP26uvw5m48XNz-uZ9PF2LCykGNTCm6ZznkOwhaYaVtpWVfakLpgRUUrIplkrDQESitIXllRCV1YaaGWUBJ2gs53c-90ozbBtTo8Kq-dmk8XatvDnItSYHm_tWc7uwn-7wCxV62LBppGd-CHqKjElFNJOE_0-we68kPo0k-S4qRgjEiR1GSnTPAxBrD7DQhW23TUNh21Tyc9-PY2dqhaqPf8PY4E-A48uAYe_zNOLW9nU0qFZK9xT5tk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2841733185</pqid></control><display><type>article</type><title>Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment</title><source>Wiley</source><creator>Durand, Benjamin A. R. N. ; Dunyach‐Remy, Catherine ; El Kaddouri, Oumayma ; Daher, Riham ; Lavigne, Jean‐Philippe ; Armengaud, Jean ; Grenga, Lucia</creator><creatorcontrib>Durand, Benjamin A. R. N. ; Dunyach‐Remy, Catherine ; El Kaddouri, Oumayma ; Daher, Riham ; Lavigne, Jean‐Philippe ; Armengaud, Jean ; Grenga, Lucia</creatorcontrib><description>Purpose
Helcococcus kunzii is a skin commensal, Gram‐positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co‐isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.
Experimental Design
The H. kunzii cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q‐Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.
Results
The H. kunzii’s cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative H. kunzii targets involved in modulating the virulence of S. aureus is investigated.
Conclusion and Clinical Relevance
This work, pioneering the study of H. kunzii physiology in a chronic wound‐like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management.</description><identifier>ISSN: 1862-8346</identifier><identifier>EISSN: 1862-8354</identifier><identifier>DOI: 10.1002/prca.202200069</identifier><identifier>PMID: 37062883</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Bacteria ; Design of experiments ; Diabetes mellitus ; exoproteome, infected wounds, mass spectrometry, opportunistic bacterium, proteome ; Growth stage ; Life Sciences ; Opportunist infection ; Pathogens ; Proteins ; Proteomes ; Proteomics ; Stationary phase ; Virulence ; Wounds</subject><ispartof>Proteomics. Clinical applications, 2023-07, Vol.17 (4), p.e2200069-n/a</ispartof><rights>2023 The Authors. Proteomics – Clinical Applications published by Wiley‐VCH GmbH.</rights><rights>2023 The Authors. Proteomics - Clinical Applications published by Wiley-VCH GmbH.</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3978-c954f3a646e5f703afba8dbac1d737b2b1838339c1e9f516bf5b5a7f8fed8e913</cites><orcidid>0000-0001-5560-1717 ; 0000-0003-1589-445X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37062883$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04459508$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Durand, Benjamin A. R. N.</creatorcontrib><creatorcontrib>Dunyach‐Remy, Catherine</creatorcontrib><creatorcontrib>El Kaddouri, Oumayma</creatorcontrib><creatorcontrib>Daher, Riham</creatorcontrib><creatorcontrib>Lavigne, Jean‐Philippe</creatorcontrib><creatorcontrib>Armengaud, Jean</creatorcontrib><creatorcontrib>Grenga, Lucia</creatorcontrib><title>Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment</title><title>Proteomics. Clinical applications</title><addtitle>Proteomics Clin Appl</addtitle><description>Purpose
Helcococcus kunzii is a skin commensal, Gram‐positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co‐isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.
Experimental Design
The H. kunzii cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q‐Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.
Results
The H. kunzii’s cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative H. kunzii targets involved in modulating the virulence of S. aureus is investigated.
Conclusion and Clinical Relevance
This work, pioneering the study of H. kunzii physiology in a chronic wound‐like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management.</description><subject>Bacteria</subject><subject>Design of experiments</subject><subject>Diabetes mellitus</subject><subject>exoproteome, infected wounds, mass spectrometry, opportunistic bacterium, proteome</subject><subject>Growth stage</subject><subject>Life Sciences</subject><subject>Opportunist infection</subject><subject>Pathogens</subject><subject>Proteins</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Stationary phase</subject><subject>Virulence</subject><subject>Wounds</subject><issn>1862-8346</issn><issn>1862-8354</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqF0c1OGzEQB3ALFTVAe-VYrdQLHBL8ues9RhE0SJGIUNur5fWOi5PddWrvguDUR-AZeRIcBXLgUvng0ejn0Vh_hE4JnhCM6cUmGD2hmFKMcV4eoCMiczqWTPBP-5rnI3Qc4wpjwWmBP6MRK3BOpWRH6Pcy-B5860zmuuj-3PUxFb3P5tAYn44ZYrYeuifnUj_TWe10BX3i1vs-GxoD4eXfc-PWkEF374LvWuj6L-jQ6ibC17f7BP26uvw5m48XNz-uZ9PF2LCykGNTCm6ZznkOwhaYaVtpWVfakLpgRUUrIplkrDQESitIXllRCV1YaaGWUBJ2gs53c-90ozbBtTo8Kq-dmk8XatvDnItSYHm_tWc7uwn-7wCxV62LBppGd-CHqKjElFNJOE_0-we68kPo0k-S4qRgjEiR1GSnTPAxBrD7DQhW23TUNh21Tyc9-PY2dqhaqPf8PY4E-A48uAYe_zNOLW9nU0qFZK9xT5tk</recordid><startdate>202307</startdate><enddate>202307</enddate><creator>Durand, Benjamin A. R. N.</creator><creator>Dunyach‐Remy, Catherine</creator><creator>El Kaddouri, Oumayma</creator><creator>Daher, Riham</creator><creator>Lavigne, Jean‐Philippe</creator><creator>Armengaud, Jean</creator><creator>Grenga, Lucia</creator><general>Wiley Subscription Services, Inc</general><general>Wiley-VCH Verlag</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5560-1717</orcidid><orcidid>https://orcid.org/0000-0003-1589-445X</orcidid></search><sort><creationdate>202307</creationdate><title>Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment</title><author>Durand, Benjamin A. R. N. ; Dunyach‐Remy, Catherine ; El Kaddouri, Oumayma ; Daher, Riham ; Lavigne, Jean‐Philippe ; Armengaud, Jean ; Grenga, Lucia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3978-c954f3a646e5f703afba8dbac1d737b2b1838339c1e9f516bf5b5a7f8fed8e913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Bacteria</topic><topic>Design of experiments</topic><topic>Diabetes mellitus</topic><topic>exoproteome, infected wounds, mass spectrometry, opportunistic bacterium, proteome</topic><topic>Growth stage</topic><topic>Life Sciences</topic><topic>Opportunist infection</topic><topic>Pathogens</topic><topic>Proteins</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Stationary phase</topic><topic>Virulence</topic><topic>Wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durand, Benjamin A. R. N.</creatorcontrib><creatorcontrib>Dunyach‐Remy, Catherine</creatorcontrib><creatorcontrib>El Kaddouri, Oumayma</creatorcontrib><creatorcontrib>Daher, Riham</creatorcontrib><creatorcontrib>Lavigne, Jean‐Philippe</creatorcontrib><creatorcontrib>Armengaud, Jean</creatorcontrib><creatorcontrib>Grenga, Lucia</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Proteomics. Clinical applications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durand, Benjamin A. R. N.</au><au>Dunyach‐Remy, Catherine</au><au>El Kaddouri, Oumayma</au><au>Daher, Riham</au><au>Lavigne, Jean‐Philippe</au><au>Armengaud, Jean</au><au>Grenga, Lucia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment</atitle><jtitle>Proteomics. Clinical applications</jtitle><addtitle>Proteomics Clin Appl</addtitle><date>2023-07</date><risdate>2023</risdate><volume>17</volume><issue>4</issue><spage>e2200069</spage><epage>n/a</epage><pages>e2200069-n/a</pages><issn>1862-8346</issn><eissn>1862-8354</eissn><abstract>Purpose
Helcococcus kunzii is a skin commensal, Gram‐positive bacterium, mostly isolated from infected chronic wounds. This opportunistic pathogen is usually co‐isolated with Staphylococcus aureus. The present dataset explores the production and secretion of H. kunzii bacterial virulence interacting proteins in a growth medium mimicking chronic wounds in exponential and stationary growth phases.
Experimental Design
The H. kunzii cellular proteome and exoproteome were assessed by analyzing three biological replicates per condition tested. Samples were analyzed using a Q‐Exactive HF mass spectrometer. Comparative and functional analyses were performed to profile the identified protein set.
Results
The H. kunzii’s cellular proteome encompassed 969 proteins, among which 64 and 53 were specifically identified in the exponential and stationary phase of growth, respectively. Its exoproteome comprised 58 proteins, among which 16 and 14 were characteristic of each growth stage. Metabolic differences between the two phases of growth are discussed. Besides, the production of previously shortlisted and novel putative H. kunzii targets involved in modulating the virulence of S. aureus is investigated.
Conclusion and Clinical Relevance
This work, pioneering the study of H. kunzii physiology in a chronic wound‐like environment, should assist future research on this opportunistic pathogen and the search for innovative approaches for wound management.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37062883</pmid><doi>10.1002/prca.202200069</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5560-1717</orcidid><orcidid>https://orcid.org/0000-0003-1589-445X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteria Design of experiments Diabetes mellitus exoproteome, infected wounds, mass spectrometry, opportunistic bacterium, proteome Growth stage Life Sciences Opportunist infection Pathogens Proteins Proteomes Proteomics Stationary phase Virulence Wounds |
| title | Proteomic insights into Helcococcus kunzii in a diabetic foot ulcer‐like environment |
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