X‐ray high‐resolution vascular network imaging

Summary This paper presents the first application of high‐resolution X‐ray synchrotron tomography to the imaging of large microvascular networks in biological tissue samples. This technique offers the opportunity of analysing the full three‐dimensional vascular network from the micrometre to the mil...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2004-08, Vol.215 (2), p.139-148
Main Authors: PLOURABOUÉ, F., CLOETENS, P., FONTA, C., STEYER, A., LAUWERS, F., MARC‐VERGNES, J.‐P.
Format: Article
Language:English
Subjects:
Animals
Blood Vessels - ultrastructure
Brain
Capillaries - ultrastructure
Cerebrovascular Circulation
contrast agent
Engineering Sciences
Humans
Image Processing, Computer-Assisted
Microcirculation - ultrastructure
microtomography
microvascular
Optics
Photonic
Rats
sample preparation
Sensitivity and Specificity
synchrotron radiation
Synchrotrons
Tomography, X-Ray - methods
X‐ray
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Summary:Summary This paper presents the first application of high‐resolution X‐ray synchrotron tomography to the imaging of large microvascular networks in biological tissue samples. This technique offers the opportunity of analysing the full three‐dimensional vascular network from the micrometre to the millimetre scale. This paper presents the specific sample preparation method and the X‐ray imaging procedure. Either barium or iron was injected as contrast agent in the vascular network. The impact of the composition and concentration of the injected solution on the X‐ray synchrotron tomography images has been studied. Two imaging modes, attenuation and phase contrast, are compared. Synchrotron high‐resolution computed tomography offers new prospects in the three‐dimensional imaging of in situ biological vascular networks.
ISSN:0022-2720
1365-2818
DOI:10.1111/j.0022-2720.2004.01362.x