The Dicentrarchus labrax estrogen screen test: A relevant tool to screen estrogen-like endocrine disrupting chemicals in the aquatic environment

In response to the need for the diversification of regulatory bioassays to screen estrogen-like endocrine disrupting chemical (EEDC) in the environment, we propose the use of a reporter gene assay involving all nuclear estrogen receptors from Dicentrarchus labrax (i.e., sbEsr1, sbEsr2a, or sbEsr2b)....

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Bibliographic Details
Published in:Chemosphere (Oxford) 2024-08, Vol.362, p.142601, Article 142601
Main Authors: Slaby, Sylvain, Duflot, Aurélie, Zapater, Cinta, Gómez, Ana, Couteau, Jérôme, Maillet, Géraldine, Knigge, Thomas, Pinto, Patrícia I.S., Monsinjon, Tiphaine
Format: Article
Language:English
Subjects:
Animal biology
Bioassay
Biodiversity and Ecology
Biomonitoring
Ecotoxicology
Effect-based tool
Endocrine disruptors
Environmental Sciences
Estrogen
Life Sciences
Sea bass
Toxicology
Vertebrate Zoology
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Summary:In response to the need for the diversification of regulatory bioassays to screen estrogen-like endocrine disrupting chemical (EEDC) in the environment, we propose the use of a reporter gene assay involving all nuclear estrogen receptors from Dicentrarchus labrax (i.e., sbEsr1, sbEsr2a, or sbEsr2b). Named DLES test (D. labrax estrogen screen), it aims at complementing existing standardized in vitro tests by implementing more estrogen receptors notably those that do not originate from humans. Positive responses were obtained with all three estrogen receptors, and—consistently with observations from other species—variations in sensitivity to E2 were measured. Sensitivity and EC50 values could be classified as follows: sbEsr2b 
ISSN:0045-6535
1879-1298
1879-1298
DOI:10.1016/j.chemosphere.2024.142601