Molecular events accompanying aggregation-induced energy quenching in fucoxanthin-chlorophyll proteins

• Published in: Biochimica et biophysica acta. Bioenergetics 2024-11, Vol.1865 (4), p.149500, Article 149500
• Main Authors: Alexandre, Maxime T.A., Krüger, Tjaart P.J., Pascal, Andrew A., Veremeienko, Vasyl, Llansola-Portoles, Manuel J., Gundermann, Kathi, van Grondelle, Rienk, Büchel, Claudia, Robert, Bruno
• Format: Article
• Language: English
• Subjects: Chemical Sciences; Diatoms; Life Sciences; Light-harvesting; NPQ; Photoprotection; Raman; Xanthophyll
• ISSN: 0005-2728; 1879-2650; 1879-2650
• DOI: 10.1016/j.bbabio.2024.149500

In high light, the antenna system in oxygenic photosynthetic organisms switches to a photoprotective mode, dissipating excess energy in a process called non-photochemical quenching (NPQ). Diatoms exhibit very efficient NPQ, accompanied by a xanthophyll cycle in which diadinoxanthin is de-epoxidized into diatoxanthin. Diatoms accumulate pigments from this cycle in high light, and exhibit faster and more pronounced NPQ. The mechanisms underlying NPQ in diatoms remain unclear, but it can be mimicked by aggregation of their isolated light-harvesting complexes, FCP (fucoxanthin chlorophyll-a/c protein). We assess this model system by resonance Raman measurements of two peripheral FCPs, trimeric FCPa and nonameric FCPb, isolated from high- and low-light-adapted cells (LL,HL). Quenching is associated with a reorganisation of these proteins, affecting the conformation of their bound carotenoids, and in a manner which is highly dependent on the protein considered. FCPa from LL diatoms exhibits significant changes in diadinoxanthin structure, together with a smaller conformational change of at least one fucoxanthin. For these LL-FCPa, quenching is associated with consecutive events, displaying distinct spectral signatures, and its amplitude correlates with the planarity of the diadinoxanthin structure. HL-FCPa aggregation is associated with a change in planarity of a 515-nm-absorbing fucoxanthin, and, to a lesser extent, of diadinoxanthin. Finally, in FCPb, a blue-absorbing fucoxanthin is primarily affected. FCPs thus possess a plastic structure, undergoing several conformational changes upon aggregation, dependent upon their precise composition and structure. NPQ in diatoms may therefore arise from a combination of structural changes, dependent on the environment the cells are adapted to. •FCP antenna proteins isolated from diatoms exhibit energy quenching when aggregated•Resonance Raman reveals that quenching correlates with specific changes in conformation of three bound carotenoids•At least five different protein conformations are required to explain the spectral changes in FCPa•The observed changes are sensitive to the light environment the diatoms were grown under