High-performance liquid chromatographic method for the determination of juglone in fresh walnut leaves

An efficient high-performance liquid chromatographic gradient method for the determination of juglone in fresh walnut leaves was developed and validated. The leaves were collected and extracted by maceration in chloroform for 1 h at room temperature. The chloroform solution was evaporated under redu...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-05, Vol.805 (1), p.315-318
Main Authors: Gı̂rzu, Mihaela, Fraisse, Didier, Carnat, André-Paul, Carnat, Andrée, Lamaison, Jean-Louis
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food and Nutrition
General pharmacology
Juglone
Life Sciences
Medical sciences
Naphthoquinones
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:An efficient high-performance liquid chromatographic gradient method for the determination of juglone in fresh walnut leaves was developed and validated. The leaves were collected and extracted by maceration in chloroform for 1 h at room temperature. The chloroform solution was evaporated under reduced pressure to give a dry residue recovered by methanol. The methanol solution was chromatographed on an LiChrospher-RP 18 end-capped 125×4 mm column, with a gradient of mobile phase: water–phosphoric acid–acetonitrile; juglone was detected by visible absorbance at 420 nm.
ISSN:0021-9673
1873-3778
DOI:10.1016/S0021-9673(97)01300-9