Altered monoterpene composition in transgenic mint following the introduction of 4S-limonene synthase

Genetic transformation of Mentha arvensis and M. x piperita with the neomycine phosphotransferase marker gene and a 4S-limonene synthase cDNA from M. spicata led to the regeneration of 47 transformed plants. In these plants, integration of the transgene was checked by PCR and Southern blots. Transcr...

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Published in:Plant physiology and biochemistry 2001-07, Vol.39 (7-8), p.603-614
Main Authors: Diemer, F. ((Université Jean Monet, Saint Etienne (France). Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales)), Caissard, J.C, Moja, S, Chalchat, J.C, Jullien, F
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Economic plant physiology
ESSENTIAL OIL CROPS
Fundamental and applied biological sciences. Psychology
Life Sciences
MENTHA ARVENSIS
MENTHA PIPERITA
Metabolism
Metabolism. Physicochemical requirements
MONOTERPENE
MONOTERPENES
MONOTERPENOS
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Plant physiology and development
PLANTAS AROMATICAS
PLANTAS TRANSGENICAS
PLANTE A HUILES ESSENTIELLES
PLANTE TRANSGENIQUE
TRANSGENIC PLANTS
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Summary:Genetic transformation of Mentha arvensis and M. x piperita with the neomycine phosphotransferase marker gene and a 4S-limonene synthase cDNA from M. spicata led to the regeneration of 47 transformed plants. In these plants, integration of the transgene was checked by PCR and Southern blots. Transcripts were detected by RT-PCR and monoterpene profiles were determined by gas chromatography. Quantitative and qualitative modifications in monoterpene levels were observed in both M. arvensis and M. x piperita transgenic plants. Four M. x piperita transgenic plants contained higher levels of total monoterpenes, whereas monoterpene levels were lower in two M. arvensis transgenic lines. In both M. x piperita and M. arvensis transgenic lines, altered levels of compounds formed directly from geranyl diphosphate such as cineole or ocimene and monoterpene end-products such as pulegone or piperitone were observed. These effects were consistent with either over-expression or co-suppression of the 4S-ls gene, depending on the transgenic line. These data demonstrate the feasibility of modifying essential oil content by the introduction of a cDNA encoding an enzyme involved in monoterpene metabolism
ISSN:0981-9428
1873-2690
0981-9428
DOI:10.1016/s0981-9428(01)01273-6