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Pri peptides temporally coordinate transcriptional programs during epidermal differentiation

To achieve a highly differentiated state, cells undergo multiple transcriptional processes whose coordination and timing are not well understood. In Drosophila embryonic epidermal cells, polished-rice (Pri) smORF peptides act as temporal mediators of ecdysone to activate a transcriptional program le...

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Published in:Science advances 2024-02, Vol.10 (6)
Main Authors: Gallois, Maylis, Menoret, Delphine, Marques-Prieto, Simon, Montigny, Audrey, Valenti, Philippe, Moussian, Bernard, Plaza, Serge, Payre, François, Chanut-Delalande, Hélène
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container_title Science advances
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creator Gallois, Maylis
Menoret, Delphine
Marques-Prieto, Simon
Montigny, Audrey
Valenti, Philippe
Moussian, Bernard
Plaza, Serge
Payre, François
Chanut-Delalande, Hélène
description To achieve a highly differentiated state, cells undergo multiple transcriptional processes whose coordination and timing are not well understood. In Drosophila embryonic epidermal cells, polished-rice (Pri) smORF peptides act as temporal mediators of ecdysone to activate a transcriptional program leading to cell shape remodeling. Here, we show that the ecdysone/Pri axis concomitantly represses the transcription of a large subset of cuticle genes to ensure proper differentiation of the insect exoskeleton. The repression relies on the transcription factor Ken and persists for several days throughout early larval stages, during which a soft cuticle allows larval crawling. The onset of these cuticle genes normally awaits the end of larval stages when the rigid pupal case assembles, and their premature expression triggers abnormal sclerotization of the larval cuticle. These results uncovered a temporal switch to set up distinct structures of cuticles adapted to the animal lifestyle and which might be involved in the evolutionary history of insects.
doi_str_mv 10.1126/sciadv.adg8816
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title Pri peptides temporally coordinate transcriptional programs during epidermal differentiation
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