Dynamic Mapping of Electrochemiluminescence Reactivity in Space: Application to Bead-Based Assays

As an electrochemical technique offering an optical readout, electrochemiluminescence (ECL) evolved recently into a powerful microscopy technique with the visualization of a wide range of microscopic entities. However, the dynamic imaging of transient ECL events did not receive intensive attention d...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-10, Vol.95 (42), p.15700-15706
Main Authors: Han, Dongni, Fang, Danjun, Valenti, Giovanni, Paolucci, Francesco, Kanoufi, Frédéric, Jiang, Dechen, Sojic, Neso
Format: Article
Language:English
Subjects:
Beads
Bioassays
Chemical Sciences
Coordination chemistry
Electrochemiluminescence
Electrochemistry
Emission analysis
Emissions
Imaging
Kinetics
Microscopy
or physical chemistry
Photons
Reaction kinetics
Theoretical and
Thickness
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Summary:As an electrochemical technique offering an optical readout, electrochemiluminescence (ECL) evolved recently into a powerful microscopy technique with the visualization of a wide range of microscopic entities. However, the dynamic imaging of transient ECL events did not receive intensive attention due to the limited number of electrogenerated photons. Here, the reaction kinetics of the model ECL bioassay system was revealed by dynamic imaging of single [Ru­(bpy)3]2+-functionalized beads in the presence of the efficient tripropylamine coreactant. The time profile behavior of ECL emission, the variations of the ECL layer thickness, and the position of maximum ECL intensity over time were investigated, which were not achieved by static imaging in previous studies. Moreover, the dynamics of the ECL emission were confronted with the simulation. The reported dynamic ECL imaging allows the investigation of the ECL kinetics and mechanisms operating in bioassays and cell microscopy.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c02960