Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics

Introduction Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. Objectives In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. Methods The c...

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Bibliographic Details
Published in:Metabolomics 2017-08, Vol.13 (8), p.1, Article 99
Main Authors: Cesbron, N., Royer, A.-L., Guitton, Y., Sydor, A., Le Bizec, B., Dervilly-Pinel, G.
Format: Article
Language:English
Subjects:
Analytical chemistry
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Centrifugation
Chemical Sciences
Developmental Biology
Dilution
Feces
Fingerprinting
Fingerprints
Life Sciences
Metabolites
Metabolomics
Methanol
Molecular Medicine
Optimization
Sample preparation
Short Communication
Solvents
Workflow
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Summary:Introduction Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. Objectives In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. Methods The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. Results A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. Conclusion The workflow generated repeatable and informative fingerprints for robust metabolome characterization.
ISSN:1573-3882
1573-3890
DOI:10.1007/s11306-017-1233-8