Transcriptomic and developmental effects of persistent organic pollutants in sentinel fishes collected near an arctic formerly used defense site

• Published in: Environmental pollution (1987) 2024-09, Vol.356, p.124283, Article 124283
• Main Authors: Jordan-Ward, Renee, von Hippel, Frank A., Sancho Santos, Maria Eugenia, Wilson, Catherine A., Rodriguez Maldonado, Zyled, Dillon, Danielle, Titus, Tom, Gardell, Alison, Salamova, Amina, Postlethwait, John H., Contreras, Elise, Capozzi, Staci L., Panuwet, Parinya, Parrocha, Chelsea, Bremiller, Ruth, Guiguen, Yann, Gologergen, Jesse, Immingan, Tiffany, Miller, Pamela, Carpenter, David, Buck, C. Loren
• Format: Article
• Language: English
• Subjects: Endocrine-disrupting compounds; Formerly used defense (FUD) site; Life Sciences; Military contamination; Persistent organic pollutants (POPs); Sivuqaq (St. Lawrence Island)
• ISSN: 0269-7491; 1873-6424; 1873-6424
• DOI: 10.1016/j.envpol.2024.124283

Alaska contains over 600 formerly used defense (FUD) sites, many of which serve as point sources of pollution. These sites are often co-located with rural communities that depend upon traditional subsistence foods, especially lipid-rich animals that bioaccumulate and biomagnify persistent organic pollutants (POPs). Many POPs are carcinogenic and endocrine-disrupting compounds that are associated with adverse health outcomes. Therefore, elevated exposure to POPs from point sources of pollution may contribute to disproportionate incidence of disease in arctic communities. We investigated PCB concentrations and the health implications of POP exposure in sentinel fishes collected near the Northeast Cape FUD site on Sivuqaq (St. Lawrence Island), Alaska. Sivuqaq residents are almost exclusively Yupik and rely on subsistence foods. At the request of the Sivuqaq community, we examined differential gene expression and developmental pathologies associated with exposure to POPs originating at the Northeast Cape FUD site. We found significantly higher levels of PCBs in Alaska blackfish (Dallia pectoralis) collected from contaminated sites downstream of the FUD site compared to fish collected from upstream reference sites. We compared transcriptomic profiles and histopathologies of these same blackfish. Blackfish from contaminated sites overexpressed genes involved in ribosomal and FoxO signaling pathways compared to blackfish from reference sites. Contaminated blackfish also had significantly fewer thyroid follicles and smaller pigmented macrophage aggregates. Conversely, we found that ninespine stickleback (Pungitius pungitius) from contaminated sites exhibited thyroid follicle hyperplasia. Despite our previous research reporting transcriptomic and endocrine differences in stickleback from contaminated vs. reference sites, we did not find significant differences in kidney or gonadal histomorphologies. Our results demonstrate that contaminants from the Northeast Cape FUD site are associated with altered gene expression and thyroid development in native fishes. These results are consistent with our prior work demonstrating disruption of the thyroid hormone axis in Sivuqaq residents. [Display omitted] •Residents of Sivuqaq live a subsistence lifestyle including at Northeast Cape (NEC).•Pollution from the NEC formerly used defense (FUD) site impacts native fishes.•Fish exposed to pollution overexpressed genes in ribosomal and FoxO pathways.•Thyroid follicle counts diffe