In vivo and ex vivo confocal microscopy for the evaluation of surgical margins of melanoma

We report the first series of melanomas (MMs) where the surgical margins were evaluated both by ex vivo confocal microscopy (EVCM) and in vivo reflectance confocal microscopy (RCM). We evaluated the surgical margins of 42 cutaneous MMs of lentigo maligna/lentigo maligna melanoma type and 2 mucosal M...

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Bibliographic Details
Published in:Journal of biophotonics 2020-11, Vol.13 (11), p.e202000179-n/a
Main Authors: Cinotti, Elisa, Belgrano, Valerio, Labeille, Bruno, Grivet, Damien, Douchet, Catherine, Chauleur, Celine, Cambazard, Frédéric, Thomas, Alix, Prade, Virginie, Tognetti, Linda, Cartocci, Alessandra, Rubegni, Pietro, Perrot, Jean Luc
Format: Article
Language:English
Subjects:
Confocal microscopy
ex vivo
Gynecology and obstetrics
Histopathology
Human health and pathology
in vivo
lentigo maligna
Life Sciences
Melanoma
Microscopes
Microscopy
Mucosa
reflectance confocal microscopy
surgery
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Summary:We report the first series of melanomas (MMs) where the surgical margins were evaluated both by ex vivo confocal microscopy (EVCM) and in vivo reflectance confocal microscopy (RCM). We evaluated the surgical margins of 42 cutaneous MMs of lentigo maligna/lentigo maligna melanoma type and 2 mucosal MMs with EVCM. Cutaneous MMs also underwent RCM mapping. Imaging results were compared with histopathology. The rate of correct identification of the tumor margins (invaded or not invaded) was 97.6% for RCM (evaluations of cutaneous MMs) and 95.5% for EVCM (evaluations of both cutaneous and mucosal MMs). Our study showed that the MM extension is visible under EVCM and that the combination of in vivo RCM and EVCM can be a new strategy for the evaluation of surgical margins of MMs. The applications of confocal microscopy are expanding in dermatology. We report the first series of melanomas (MMs) where the surgical margins were evaluated both by in vivo and ex vivo confocal microscopy and we found a good correlation with histopathology. These techniques could help to reduce surgical stages and local recurrences after surgery especially in clinically ill‐defined MMs such as lentigo maligna.
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.202000179