Pegylated liposome encapsulating docetaxel using microfluidic mixing technique: Process optimization and results in breast cancer models

• Published in: International journal of pharmaceutics 2024-05, Vol.656, p.124091-124091, Article 124091
• Main Authors: Dacos, Mathilde, Immordino, Benoît, Diroff, Erwan, Sicard, Guillaume, Kosta, Artemis, Rodallec, Anne, Giacometti, Sarah, Ciccolini, Joseph, Fanciullino, Raphaëlle
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacokinetics; Biopharmaceutical development; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Cell Line, Tumor; Cell Proliferation - drug effects; Docetaxel; Docetaxel - administration & dosage; Docetaxel - chemistry; Docetaxel - pharmacokinetics; Female; Humans; Life Sciences; Liposomes; Mice; Mice, Inbred C57BL; Microfluidic mixing technique; Microfluidics - methods; Particle Size; Pharmacokinetics; Polyethylene Glycols - chemistry
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2024.124091

[Display omitted] The development of nanoparticles could help to improve the efficacy/toxicity balance of drugs. This project aimed to develop liposomes and immunoliposomes using microfluidic mixing technology.Various formulation tests were carried out to obtain liposomes that met the established specifications. The liposomes were then characterized in terms of size, polydispersity index (PDI), docetaxel encapsulation rate and lamellarity. Antiproliferative activity was tested in human breast cancer models ranging from near-negative (MDA-MB-231), positive (MDA-MB-453) to HER2 positive. Pharmacokinetic studies were performed in C57BL/6 mice.Numerous batches of liposomes were synthesised using identical molar ratios and by varying the microfluidic parameters TFR, FRR and buffer. All synthesized liposomes have a size