Electrospun Honeycomb as Nests for Controlled Osteoblast Spatial Organization

• Published in: Macromolecular bioscience 2014-11, Vol.14 (11), p.1580-1589
• Main Authors: Nedjari, Salima, Eap, Sandy, Hébraud, Anne, Wittmer, Corinne R., Benkirane-Jessel, Nadia, Schlatter, Guy
• Format: Article
• Language: English
• Subjects: Biotechnology; bone regeneration; Cell Adhesion - drug effects; Cell Line; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell Proliferation - drug effects; Controllers; Electrospinning; Fibers; Honeycomb; Honeycomb construction; Humans; Lactic Acid - pharmacology; Life Sciences; micro-structured scaffolds; Microscopy, Fluorescence; Nanofibers - ultrastructure; Nanostructure; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - ultrastructure; osteoblasts-like cells; Polyesters - pharmacology; Polymers - pharmacology; Programmable logic devices; Scaffolds; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201400226

Honeycomb nanofibrous scaffolds were elaborated by electrospinning onto micro‐patterned collectors either with poly(ϵ‐caprolactone) (PCL) or poly(D, L‐lactic acid) (PLA). The unimodal distribution of fiber diameters, observed for PLA, led to relatively flat scaffolds; on the other hand, the bimodal distribution of PCL fiber diameters significantly increased the relief of the scaffolds' patterns due to the preferential deposition of the thick fiber portions on the walls of the collector's patterns via preferential electrostatic interaction. Finally, a biological evaluation demonstrated the effect of the scaffolds' relief on the spatial organization of MG63 osteoblast‐like cells. Mimicking hemi‐osteons, cell gathering was observed inside PCL honeycomb nests with a size ranging from 80 to 360 µm. PCL and PLA nanofibrous scaffolds with honeycomb micro‐patterns are prepared by electrospinning. It is shown that the relief of the scaffolds depends on the distribution of fiber diameters. Mimicking hemi‐osteons, cell gathering is observed inside the PCL honeycomb fibrous nests with a characteristic size greater than 80 μm.