Improvement of Stability and Cell Adhesion Properties of Polyelectrolyte Multilayer Films by Chemical Cross-Linking

Poly(l-lysine)/hyaluronan (PLL/HA) films were chemically cross-linked with a water soluble carbodiimide (EDC) in combination with a N-hydroxysulfo-succinimide (NHS) to induce amide formation. Fourier transform infrared spectroscopy confirms the conversion of carboxylate and ammonium groups into amid...

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Bibliographic Details
Published in:Biomacromolecules 2004-03, Vol.5 (2), p.284-294
Main Authors: Richert, Ludovic, Boulmedais, Fouzia, Lavalle, Philippe, Mutterer, Jérôme, Ferreux, Emmanuelle, Decher, Gero, Schaaf, Pierre, Voegel, Jean-Claude, Picart, Catherine
Format: Article
Language:English
Subjects:
Aminoacid polymers
Applied sciences
Biochemistry, Molecular Biology
Cell Adhesion
Cell Line, Tumor
Cross-Linking Reagents - chemistry
Cross-Linking Reagents - metabolism
Drug Stability
Elasticity
Ethyldimethylaminopropyl Carbodiimide - chemistry
Ethyldimethylaminopropyl Carbodiimide - metabolism
Exact sciences and technology
Humans
Hyaluronic Acid - chemistry
Hyaluronic Acid - metabolism
Life Sciences
Microscopy, Confocal
Physicochemistry of polymers
Polylysine - chemistry
Polylysine - metabolism
Solutions
Spectroscopy, Fourier Transform Infrared
Synthetic biopolymers
Tissue Adhesives - chemistry
Viscosity
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Summary:Poly(l-lysine)/hyaluronan (PLL/HA) films were chemically cross-linked with a water soluble carbodiimide (EDC) in combination with a N-hydroxysulfo-succinimide (NHS) to induce amide formation. Fourier transform infrared spectroscopy confirms the conversion of carboxylate and ammonium groups into amide bonds. Quartz crystal microbalance-dissipation reveals that the cross linking reaction is accompanied by a change in the viscoelastic properties of the films leading to more rigid films. After the cross-linking reaction, both positively and negatively ending films exhibit a negative zeta potential. It is shown by fluorescence recovery after photobleaching measured by confocal laser scanning microscopy that cross-linking dramatically reduces the diffusion of the PLL chains in the network. Cross linking also renders the films highly resistant to hyaluronidase, an enzyme that naturally degrades hyaluronan. Finally, the adhesion of chondrosarcoma cells on the films terminating either with PLL or HA is also investigated. Whereas the non cross-linked films are highly resistant to cell adhesion, the cells adhere and spread well on the cross-linked films.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm0342281