Versatile primers for the amplification of the mitochondrial DNA control region in marsupials

The noncoding control region (also called 'D-loop region' in vertebrates) is the most rapidly evolving portion of the mitochondrial genome and is thus well suited for detection of nucleotide polymorphism in closely related taxa and for determining intraspecific molecular population structu...

Full description

Saved in:
Bibliographic Details
Published in:Molecular ecology 1997-12, Vol.6 (12), p.1199-1201
Main Authors: Fumagalli, Luca, Pope, Lisa C., Taberlet, Pierre, Moritz, Craig
Format: Article
Language:English
Subjects:
Animals
Biodiversity
Biodiversity and Ecology
DNA Primers
DNA, Mitochondrial - genetics
Ecology, environment
Environmental Sciences
Life Sciences
Marsupialia - genetics
Polymerase Chain Reaction - methods
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The noncoding control region (also called 'D-loop region' in vertebrates) is the most rapidly evolving portion of the mitochondrial genome and is thus well suited for detection of nucleotide polymorphism in closely related taxa and for determining intraspecific molecular population structure. Within the control region and its flanking tRNA genes there are several conserved sequence blocks, and different sets of universal primers have been designed by comparing these sequences in a wide range of taxa. However, these primers were designed from DNA sequences of placental mammals and other vertebrate species, and when used on marsupials we had limited success. In this study we describe four new primers which allow the amplification of the marsupial mtDNA control region.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294X.1997.00298.x