New antibacterial and cytotoxic activities of falcarindiol isolated in Crithmum maritimum L. leaf extract

• Published in: Food and chemical toxicology 2010-02, Vol.48 (2), p.553-557
• Main Authors: Meot-Duros, Laetitia, Cérantola, Stéphane, Talarmin, Hélène, Le Meur, Christophe, Le Floch, Gaëtan, Magné, Christian
• Format: Article
• Language: English
• Subjects: Animals; Antibacterial; antibacterial properties; antibiotics; Apiaceae; Apiaceae - chemistry; Bacillus cereus; Bacillus cereus - drug effects; Bacillus cereus - growth & development; bioactive properties; Biological and medical sciences; biopesticides; Cell Line; Cell Survival - drug effects; Chemical Fractionation; Crithmum; Crithmum maritimum; Crithmum maritimum L; Cytotoxicity; Diynes - analysis; Diynes - pharmacology; Enterocytes - drug effects; Enterocytes - metabolism; Enterocytes - pathology; Falcarindiol; Fatty Alcohols - analysis; Fatty Alcohols - pharmacology; food preservatives; Formazans - metabolism; General pharmacology; halophytes; Intestine, Small; leaves; Magnetic Resonance Spectroscopy; Medical sciences; medicinal plants; Microbial Sensitivity Tests; Micrococcus luteus; Micrococcus luteus - drug effects; Micrococcus luteus - growth & development; minimum inhibitory concentration; new products; nuclear magnetic resonance spectroscopy; perennials; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; plant extracts; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plants, Medicinal - chemistry; Rats; structure-activity relationships; Tetrazolium Salts - metabolism
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.11.031

Bioassay-guided fractionation of a chloroformic extract obtained from Crithmum maritimum leaves led to the chemical isolation of falcarindiol, a polyacetylene widely distributed within the Apiaceae family. Structure of this compound was confirmed by NMR and activity was tracked using a screening microplate bioassay. Falcarindiol strongly inhibited the growth of Micrococcus luteus and Bacillus cereus, with a minimum inhibitory concentration (MIC) value of 50 μg ml −1. Moreover, this compound showed cytotoxicity against IEC-6 cells with an IC 50 value of 20 μM after 48 h of exposition. These results suggest that Crithmum maritimum could be potentially used in food manufactures and cosmetology as preservative agents and biopesticides, or in medicine as new antibiotics, confirming the interest in studying halophytic species as sources of bioactive compounds.