Radio-imaging for quantitative autoradiography in biology

We present here an overview of new in vitro and ex vivo radio-imaging systems developed to overcome the limitations of films and emulsions currently used in histological autoradiography experiments. The shortcomings of films for quantitative studies are first introduced. Principles and performances...

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Bibliographic Details
Published in:Nuclear medicine and biology 1998-11, Vol.25 (8), p.699-704
Main Authors: Charon, Y, Lanièce, P, Tricoire, H
Format: Article
Language:English
Subjects:
Autoradiography
Autoradiography - methods
Biological and medical sciences
Biological Physics
Immunohistochemistry
In Situ Hybridization
Investigative techniques, diagnostic techniques (general aspects)
Isotope Labeling
Luminescent Measurements
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Physics
Quantitation
Radio-imaging
Radiographic Image Enhancement - instrumentation
Radiographic Image Interpretation, Computer-Assisted
Radionuclide Imaging - instrumentation
Receptor binding
X-Ray Film
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Summary:We present here an overview of new in vitro and ex vivo radio-imaging systems developed to overcome the limitations of films and emulsions currently used in histological autoradiography experiments. The shortcomings of films for quantitative studies are first introduced. Principles and performances of each family of imagers are discussed and illustrated in various biological contexts. Finally, perspectives of development including nonradioactive labeling techniques are briefly presented.
ISSN:0969-8051
1872-9614
DOI:10.1016/S0969-8051(98)00052-3