Matrix Implanted Laser Desorption Ionization (MILDI) Combined with Ion Mobility-Mass Spectrometry for Bio-Surface Analysis

The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up...

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Bibliographic Details
Published in:Journal of proteome research 2005-03, Vol.4 (2), p.540-545
Main Authors: Tempez, A, Ugarov, M, Egan, T, Schultz, J. A, Novikov, A, Della-Negra, S, Lebeyec, Y, Pautrat, M, Caroff, M, Smentkowski, V. S, Wang, H-Y. J, Jackson, S. N, Woods, A. S
Format: Article
Language:English
Subjects:
Animals
Biological Physics
Brain Chemistry
Lipids - chemistry
Molecular Weight
Peptides - chemistry
Physics
Rats
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Surface Properties
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Summary:The implantation of low velocity massive gold cluster ions allows homogeneous incorporation of a metallic matrix into the near-surface region of rat brain tissues. Subsequent analysis by laser desorption ionization mass spectrometry yields spectra exhibiting molecular ion peaks in the mass range up to 35 kDa similar to those observed by matrix-assisted LDI. Matrix-implanted LDI when combined with ion-mobility preseparation promises to be a useful technique for molecular imaging of biotissues with a laser microprobe. Keywords: matrix implanted laser desorption ionization • mass spectrometry • gold • tissue • ion mobility
ISSN:1535-3893
1535-3907
DOI:10.1021/pr0497879