Physico-chemical–mechanical and in vitro biological properties of calcium phosphate cements with doped amorphous calcium phosphates

• Published in: Biomaterials 2007-02, Vol.28 (6), p.956-965
• Main Authors: Julien, Marion, Khairoun, Ibrahim, LeGeros, Racquel Z, Delplace, Severine, Pilet, Paul, Weiss, Pierre, Daculsi, Guy, Bouler, Jean Michel, Guicheux, Jerome
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Advanced Basic Science; Amorphous; Animals; Biocompatibility; Bioengineering; Biomaterials; Bone Cements; Bone Cements - chemistry; Bone Cements - pharmacology; Calcium phosphate cements; Calcium Phosphates; Calcium Phosphates - administration & dosage; Calcium Phosphates - chemistry; Cell Size; Cell Size - drug effects; Cell Survival; Cell Survival - drug effects; Dentistry; Hardness; Life Sciences; Macroporous; Materials Testing; Mice; Molecular Conformation; Osteoblasts; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - physiology; Particle Size; Porosity; Surface Properties
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2006.10.018

Abstract Calcium phosphate cements (CPCs) are successfully used as bone substitutes in dentistry and orthopaedic applications. This study investigated the physico-chemical–mechanical properties of and in vitro biological properties (cell response) of CPCs prepared with amorphous calcium carbonate phosphate (ACCP) doped with magnesium (ACCP-Mg), zinc (ACCp-Zn) or fluoride (ACCP-F) ions. The experimental CPC consisted of α -TCP, doped ACCP, and MPCM powders as matrix and biphasic calcium phosphate (BCP) granules. X-ray diffraction analysis showed that the matrix converted to apatite with poor crystallinity (reflecting small crystal size) after setting for 24 h, while BCP remained apparently unchanged. Cements with ACCP-F (F-CPC) had shorter setting times and greater compressive strength compared to cements with ACCP-Mg (Mg-CPC) or ACCP-Zn (Zn-CPC). Scanning electron microscopy (SEM) showed that crystals set on Mg-CPC and Zn-CPC were smaller compared to those on F-CPC. The total porosity of Mg-CPC was greater compared to Zn-CPC or F-CPC. Osteoblast-like cells, MC3T3-E1, remained viable and maintained their ability to express alkaline phosphatase in contact with the CPCs with doped ACCPs.