Effect of chronic renal failure with metabolic acidosis on alanine metabolism in isolated liver cells

Background & aims: Decreased ureagenesis and gluconeogenesis from alanine have been reported during chronic renal failure in rat. This study addressed the respective roles of plasma-membrane transport and intracellular metabolism in these abnormalities of alanine pathways. Methods: In hepatocyte...

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Bibliographic Details
Published in:Clinical nutrition (Edinburgh, Scotland) Scotland), 2004-06, Vol.23 (3), p.317-324
Main Authors: Cano, N, Sturm, J.M, Meijer, A.J, El-Mir, M.Y, Novaretti, R, Reynier, J.P, Leverve, X.M
Format: Article
Language:English
Subjects:
Acidosis
Acidosis - complications
Acidosis - metabolism
Alanine
Alanine - metabolism
Amino acid metabolism
Animals
Biological and medical sciences
Cells, Cultured
Chronic renal failure
Gluconeogenesis
Gluconeogenesis - physiology
Hepatocyte
Hepatocytes
Hepatocytes - metabolism
Kidney Failure, Chronic
Kidney Failure, Chronic - complications
Kidney Failure, Chronic - metabolism
Liver
Liver - cytology
Liver - metabolism
Male
Medical sciences
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Rats
Rats, Wistar
Renal failure
Urea
Urea - metabolism
Uremia
Uremia - metabolism
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Summary:Background & aims: Decreased ureagenesis and gluconeogenesis from alanine have been reported during chronic renal failure in rat. This study addressed the respective roles of plasma-membrane transport and intracellular metabolism in these abnormalities of alanine pathways. Methods: In hepatocytes isolated from uremic and control rats, we investigated: (1) the influence of uremia on gluconeogenesis and ureagenesis during incubations with alanine; (2) the kinetics of alanine plasma-membrane transport; (3) the relationships between intracellular alanine concentrations and its metabolism. Plasma-membrane alanine transport was assessed after addition of alanine (2 mM) by measuring its intracellular accumulation from 0 to 10 min, in the presence of a transaminase inhibitor. Alanine metabolism was studied in perifused hepatocytes by measuring intracellular alanine concentration together with urea, glucose and lactate production in the presence of increasing concentrations of alanine (0–8 mM). Results: Uremic rats showed decreased plasma bicarbonate. Uremia induced ( P
ISSN:0261-5614
1532-1983
DOI:10.1016/j.clnu.2003.07.004