Comment on "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake"

Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2007-02, Vol.315 (5813), p.766; author reply 766-766
Main Authors: Chartrel, N, Alvear-Perez, R, Leprince, J, Iturrioz, X, Reaux-Le Goazigo, A, Audinot, V, Chomarat, P, Coge, F, Nosjean, O, Rodriguez, M, Galizzi, J P, Boutin, J A, Vaudry, H, Llorens-Cortes, C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Calcium
Calcium - metabolism
Cell Membrane
Cell Membrane - metabolism
CHO Cells
Colforsin - pharmacology
Cricetinae
Cricetulus
Cyclic AMP
Cyclic AMP - metabolism
Endocrinology and metabolism
Forskolin
Ghrelin
Human health and pathology
Humans
Life Sciences
Ligands
Molecular Sequence Data
Peptide Hormones
Peptide Hormones - genetics
Peptide Hormones - metabolism
Peptide Hormones - pharmacology
Pituitary Gland
Pituitary Gland - cytology
Pituitary Gland - metabolism
Protein Binding
Receptors, G-Protein-Coupled
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - metabolism
Transfection
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Summary:Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected cells in various functional assays (cyclic adenosine monophosphate production, calcium mobilization, and GPR39 internalization). Our results indicate that obestatin is not the cognate ligand for GPR39.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1135047