Enterocytic differentiation is modulated by lipid rafts-dependent assembly of adherens junctions: lipid raft and enterocytic differentiation

Integrity of the epithelial barrier is determined by apical junctional complexes which also participate in the signalling pathways inducing intestinal cell differentiation. Lipid rafts (LR) have been proposed to play a role in the organization and the function of these intercellular complexes. This...

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Bibliographic Details
Published in:Experimental cell research 2011-06, Vol.317 (10), p.1422-36
Main Authors: Chartier, Nicolas T., Lainé, Michèle G., Ducarouge, Benjamin, Oddou, Christiane, Bonaz, Bruno, Albiges-Rizo, Corinne, Jacquier-Sarlin, Muriel R.
Format: Article
Language:English
Subjects:
Adherens Junctions
Animals
Apoptosis
Blotting, Western
Cadherins
Cell Differentiation
Cell Proliferation
Cellular Biology
Enterocytes
Fluorescent Antibody Technique
HT29 Cells
Human health and pathology
Humans
Hépatology and Gastroenterology
Immunoenzyme Techniques
Immunoprecipitation
Life Sciences
Membrane Microdomains
Membrane Proteins
p120 GTPase Activating Protein
rac1 GTP-Binding Protein
Rats
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Summary:Integrity of the epithelial barrier is determined by apical junctional complexes which also participate in the signalling pathways inducing intestinal cell differentiation. Lipid rafts (LR) have been proposed to play a role in the organization and the function of these intercellular complexes. This study investigated potential mechanisms by which LR could participate in the establishment of adherens junctions (AJ) and the initiation of enterocytic differentiation. We showed that the differentiation of epithelial cells in rat colons correlates with the emergence of LR. Using HT-29 cells we demonstrated that during the differentiation process, LR are required for the recruitment and the association of p120ctn to E-cadherin. Silencing of flotillin-1, a LR component, alters the recruitment of AJ proteins in LR and delays the expression of differentiation markers. Furthermore, the ability of p120ctn/E-cadherin complexes to support cell differentiation is altered in HT-29 Rac1N17 cells. These results show a contributory role of LR in the enterocytic differentiation process, which serve as signalling platforms for Rac1-mediated organization of AJ. A better understanding of the mechanism involved in the establishment of junctional complex and their role in enterocytic differentiation provides new insights into the regulation of intestinal homeostasis.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2011.03.009