Reconstitution of the entire hepatitis C virus life cycle in non-hepatic cells: HCV life cycle in non-hepatic cells

Hepatitis C virus (HCV) is a human hepatotropic virus, yet the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human non-hepatic 293T cells. This...

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Bibliographic Details
Published in:Journal of virology 2012-08
Main Authors: da Costa, Daniel, Turek, Marine, Felmlee, Daniel J., Girardi, Erika, Pfeffer, Sébastien, Long, Gang, Bartenschlager, Ralf, Zeisel, Mirjam B., Baumert, Thomas F.
Format: Article
Language:English
Subjects:
Human health and pathology
Hépatology and Gastroenterology
Life Sciences
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Summary:Hepatitis C virus (HCV) is a human hepatotropic virus, yet the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human non-hepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human non-hepatic cells, and highlights key host factors required for liver tropism of HCV.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.01066-12