Molecular and expression analysis of the Allograft inflammatory factor 1 (AIF-1) in the coelomocytes of the common sea urchin Paracentrotus lividus

Allograft inflammatory factor 1 (AIF-1) is a highly conserved gene involved in inflammation, cloned and characterized in several evolutionary distant animal species. Here, we report the molecular identification, characterization and expression of AIF-1 from the common sea urchin Paracentrotus lividu...

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Bibliographic Details
Published in:Fish & shellfish immunology 2017-12, Vol.71, p.136-143
Main Authors: Barca, Amilcare, Vacca, Francesca, Vizioli, Jacopo, Drago, Francesco, Vetrugno, Carla, Verri, Tiziano, Pagliara, Patrizia
Format: Article
Language:English
Subjects:
Allograft inflammatory factor 1
Amino Acid Sequence
Amoebocytes
Animals
Bacterial challenge
Base Sequence
Calcium-Binding Proteins - chemistry
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - immunology
Coelomocytes
Gene Expression Profiling
Gene Expression Regulation - immunology
Immunity, Innate - genetics
Life Sciences
Paracentrotus - genetics
Paracentrotus - immunology
Paracentrotus lividus
Phylogeny
Red cells
Sequence Alignment
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Summary:Allograft inflammatory factor 1 (AIF-1) is a highly conserved gene involved in inflammation, cloned and characterized in several evolutionary distant animal species. Here, we report the molecular identification, characterization and expression of AIF-1 from the common sea urchin Paracentrotus lividus. In this species, AIF-1 encodes a predicted 151 amino acid protein with high similarity to vertebrate AIF-1 proteins. Immunocytochemical analyses on coelomocytes reveal localization of the AIF-1 protein in amoebocytes (perinuclear cytoplasmic zone) and red sphaerulocytes (inside granules), but not in vibratile cells and colorless sphaerula cells. The significant increase of AIF-1 expression (mRNA and protein) found in the coelomocytes of the sea urchin after Gram + bacterial challenge suggests the involvement of AIF-1 in the inflammatory response. Our analysis on P. lividus AIF-1 contributes to elucidate AIF-1 function along the evolutionary scale and consolidate the key evolutionary position of echinoderms throughout metazoans with respect to the common immune paths. •A gene from Paracentrotus lividus encoding the Allograft inflammatory factor1 was identified.•Expression of AIF-1 was identified in coelomocytes and changed 24 h after immune challenge.•The AIF-1 protein showed different intracellular localization depending on the coelomocytes type.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2017.09.078