Portal Glucose Infusion, Afferent Nerve Fibers, and Glucose and Insulin Tolerance of Insulin-Resistant Rats

• Published in: The Journal of nutrition 2022-08, Vol.152 (8), p.1862-1871
• Main Authors: Joly-Amado, Aurélie, Soty, Maud, Philippe, Erwann, Lacombe, Amelie, Castel, Julien, Pillot, Bruno, Vily-Petit, Justine, Zitoun, Carine, Mithieux, Gilles, Magnan, Christophe
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Blood Glucose - metabolism; Brain; Capsaicin; Capsaicin - metabolism; Capsaicin - pharmacology; Carbohydrates; Carotid arteries; Carotid artery; Diabetes; Diet; Emulsions - metabolism; Glucose; Glucose - metabolism; Glucose tolerance; hepatoportal glucose sensor; High fat diet; Insulin; Insulin - metabolism; Insulin Resistance; Insulin, Regular, Human - pharmacology; Life Sciences; Lipids; Liver; Liver - metabolism; Male; Nerve Fibers - metabolism; Nerves; Neurons and Cognition; Portal vein; Portal Vein - metabolism; Proteins; Rats; Rats, Wistar; Rodents; Sensors; sensory nerves; Sensory neurons; Triglycerides; Triglycerides - metabolism
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/nxac097

The role of hepatoportal glucose sensors is poorly understood in the context of insulin resistance. We assessed the effects of glucose infusion in the portal vein on insulin tolerance in 2 rat models of insulin resistance, and the role of capsaicin sensitive nerves in this signal. Male Wistar rats, 8 weeks old, weighing 250–275 g, were used. Insulin and glucose tolerance were assessed following a 4-hour infusion of either glucose or saline through catheterization in the portal vein in 3 paradigms. In experiment 1, for diet-induced insulin resistance, rats were fed either a control diet (energy content: proteins = 22.5%, carbohydrates = 64.1%, and lipids = 13.4%) or a high-fat diet (energy content: proteins = 15.3%, carbohydrates = 40.3%, and lipids =44.4%) for 4 months. In experiment 2, for centrally induced peripheral insulin resistance, catheters were inserted in the carotid artery to deliver either an emulsion of triglycerides [intralipid (IL)] or saline towards the brain for 24 hours. In experiment 3, for testing the role of capsaicin-sensitive nerves, experiment 2 was repeated following a periportal treatment with capsaicin or vehicle. In experiment 1, when compared to rats fed the control diet, rats fed the high-fat diet exhibited decreased insulin and glucose tolerance (P ≤ 0.05) that was restored with a glucose infusion in the portal vein (P ≤ 0.05). In experiment 2, infusion of a triglyceride emulsion towards the brain (IL rats) decreased insulin and glucose tolerance and increased hepatic endogenous production when compared to saline-infused rats (P ≤ 0.05). Glucose infusion in the portal vein in IL rats restored insulin and glucose tolerance, as well as hepatic glucose production, to controls levels (P ≤ 0.05). In experiment 3, portal infusion of glucose did not increase insulin tolerance in IL rats that received a periportal pretreatment with capsaicin. Stimulation of hepatoportal glucose sensors increases insulin tolerance in rat models of insulin resistance and requires the presence of capsaicin-sensitive nerves.