Effects of the Two Somatostatin Variants Somatostatin-14 and [Pro2, Met13]Somatostatin-14 on Receptor Binding, Adenylyl Cyclase Activity and Growth Hormone Release from the Frog Pituitary

• Published in: Journal of neuroendocrinology 1998-03, Vol.10 (3), p.187-192
• Main Authors: Jeandel, L, Okuno, A, Kobayashi, T, Kikuyama, S, Tostivint, H, Lihrmann, I, Chartrel, N, Conlon, J M, Fournier, A, Tonon, M C, Vaudry, H
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclases - metabolism; Amino Acid Sequence; Animals; Cyclic AMP - biosynthesis; Hormone Antagonists - pharmacology; Human Growth Hormone - metabolism; Life Sciences; Molecular Sequence Data; Neurons and Cognition; Pituitary Gland - metabolism; Rana catesbeiana; Rana ridibunda; Receptors, Somatostatin - drug effects; Receptors, Somatostatin - metabolism; Somatostatin - analogs & derivatives; Somatostatin - genetics; Somatostatin - pharmacology
• ISSN: 0953-8194; 1365-2826
• DOI: 10.1046/j.1365-2826.1998.00188.x

Two isoforms of somatostatin from frog brain have been recently characterized, namely somatostatin‐14 (SS1) and [Pro2, Met13]somatostatin‐14 (SS2). The genes encoding for the precursors of these two somatostatin variants are expressed in hypothalamic nuclei involved in the control of the frog pituitary. The aim of the present study was to investigate the effect of SS1 and SS2 on adenohypophysial cells. Autoradiographic studies using [125I‐Tyr°, d‐Trp8] SS1 as a radioligand revealed that somatostatin binding sites are evenly distributed in the frog pars distalis. The SS2 variant was significantly (P