Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6

Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, Fr...

Full description

Saved in:
Bibliographic Details
Published in:The Lancet (British edition) 2005-12, Vol.366 (9501), p.1960-3
Main Authors: Jambou, Ronan, Legrand, Eric, Niang, Makhtar, Khim, Nimol, Lim, Pharath, Volney, Béatrice, Ekala, Marie Thérèse, Bouchier, Christiane, Esterre, Philippe, Fandeur, Thierry, Mercereau-Puijalon, Odile
Format: Article
Language:English
Subjects:
Animals
Antimalarials
Artemisinins
Drug Resistance, Multiple
Genotype
Humans
Life Sciences
Microbiology and Parasitology
Parasitic Sensitivity Tests
Parasitology
Plasmodium falciparum
Point Mutation
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 3
container_issue 9501
container_start_page 1960
container_title The Lancet (British edition)
container_volume 366
creator Jambou, Ronan
Legrand, Eric
Niang, Makhtar
Khim, Nimol
Lim, Pharath
Volney, Béatrice
Ekala, Marie Thérèse
Bouchier, Christiane
Esterre, Philippe
Fandeur, Thierry
Mercereau-Puijalon, Odile
description Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (>30 nmol/L) artemether IC50s (p
doi_str_mv 10.1016/S0140-6736(05)67787-2
format article
fullrecord <record><control><sourceid>hal</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_pasteur_00590989v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_HAL_pasteur_00590989v1</sourcerecordid><originalsourceid>FETCH-hal_primary_oai_HAL_pasteur_00590989v13</originalsourceid><addsrcrecordid>eNqVjT1rwzAURUVpadyPn1B4YzuofYplyR5NSMnQwSQZuplHLBMV2zKSHPC_bwKle6Z74B7uZexF4LtAoT52KCRypVP1itmb0jrXfHnDEiG15JnU37cs-VcW7CGEH0SUCrN7thAqXWaqyBM2b02wIdJwMOBaqDoKvWvs1ENL3cGO5C9oTdeADa6jaAJEB3bgJxu9A_LR9CYejQcaGhidHSL0U6Ro3RAuk-cOduvtquRxHg1UbbmvKBj1xO7OF8E8_-Uj45_r_WrDj9TVo7c9-bl2ZOtN-VWPFKKZfI2YFVjkxUmk1_q_n5NeVA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6</title><source>Business Source Ultimate</source><source>Elsevier</source><creator>Jambou, Ronan ; Legrand, Eric ; Niang, Makhtar ; Khim, Nimol ; Lim, Pharath ; Volney, Béatrice ; Ekala, Marie Thérèse ; Bouchier, Christiane ; Esterre, Philippe ; Fandeur, Thierry ; Mercereau-Puijalon, Odile</creator><creatorcontrib>Jambou, Ronan ; Legrand, Eric ; Niang, Makhtar ; Khim, Nimol ; Lim, Pharath ; Volney, Béatrice ; Ekala, Marie Thérèse ; Bouchier, Christiane ; Esterre, Philippe ; Fandeur, Thierry ; Mercereau-Puijalon, Odile</creatorcontrib><description>Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (&gt;30 nmol/L) artemether IC50s (p&lt;0.0001, Mann-Whitney). All resistant isolates came from areas with uncontrolled use of artemisinin derivatives. This rise in resistance indicates the need for increased vigilance and a coordinated and rapid deployment of drug combinations.</description><identifier>ISSN: 0140-6736</identifier><identifier>EISSN: 1474-547X</identifier><identifier>DOI: 10.1016/S0140-6736(05)67787-2</identifier><identifier>PMID: 16325698</identifier><language>eng</language><publisher>Elsevier</publisher><subject>Animals ; Antimalarials ; Artemisinins ; Drug Resistance, Multiple ; Genotype ; Humans ; Life Sciences ; Microbiology and Parasitology ; Parasitic Sensitivity Tests ; Parasitology ; Plasmodium falciparum ; Point Mutation</subject><ispartof>The Lancet (British edition), 2005-12, Vol.366 (9501), p.1960-3</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-3549-1543 ; 0000-0003-3549-1543</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://riip.hal.science/pasteur-00590989$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Jambou, Ronan</creatorcontrib><creatorcontrib>Legrand, Eric</creatorcontrib><creatorcontrib>Niang, Makhtar</creatorcontrib><creatorcontrib>Khim, Nimol</creatorcontrib><creatorcontrib>Lim, Pharath</creatorcontrib><creatorcontrib>Volney, Béatrice</creatorcontrib><creatorcontrib>Ekala, Marie Thérèse</creatorcontrib><creatorcontrib>Bouchier, Christiane</creatorcontrib><creatorcontrib>Esterre, Philippe</creatorcontrib><creatorcontrib>Fandeur, Thierry</creatorcontrib><creatorcontrib>Mercereau-Puijalon, Odile</creatorcontrib><title>Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6</title><title>The Lancet (British edition)</title><description>Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (&gt;30 nmol/L) artemether IC50s (p&lt;0.0001, Mann-Whitney). All resistant isolates came from areas with uncontrolled use of artemisinin derivatives. This rise in resistance indicates the need for increased vigilance and a coordinated and rapid deployment of drug combinations.</description><subject>Animals</subject><subject>Antimalarials</subject><subject>Artemisinins</subject><subject>Drug Resistance, Multiple</subject><subject>Genotype</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Parasitic Sensitivity Tests</subject><subject>Parasitology</subject><subject>Plasmodium falciparum</subject><subject>Point Mutation</subject><issn>0140-6736</issn><issn>1474-547X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqVjT1rwzAURUVpadyPn1B4YzuofYplyR5NSMnQwSQZuplHLBMV2zKSHPC_bwKle6Z74B7uZexF4LtAoT52KCRypVP1itmb0jrXfHnDEiG15JnU37cs-VcW7CGEH0SUCrN7thAqXWaqyBM2b02wIdJwMOBaqDoKvWvs1ENL3cGO5C9oTdeADa6jaAJEB3bgJxu9A_LR9CYejQcaGhidHSL0U6Ro3RAuk-cOduvtquRxHg1UbbmvKBj1xO7OF8E8_-Uj45_r_WrDj9TVo7c9-bl2ZOtN-VWPFKKZfI2YFVjkxUmk1_q_n5NeVA</recordid><startdate>20051203</startdate><enddate>20051203</enddate><creator>Jambou, Ronan</creator><creator>Legrand, Eric</creator><creator>Niang, Makhtar</creator><creator>Khim, Nimol</creator><creator>Lim, Pharath</creator><creator>Volney, Béatrice</creator><creator>Ekala, Marie Thérèse</creator><creator>Bouchier, Christiane</creator><creator>Esterre, Philippe</creator><creator>Fandeur, Thierry</creator><creator>Mercereau-Puijalon, Odile</creator><general>Elsevier</general><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3549-1543</orcidid><orcidid>https://orcid.org/0000-0003-3549-1543</orcidid></search><sort><creationdate>20051203</creationdate><title>Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6</title><author>Jambou, Ronan ; Legrand, Eric ; Niang, Makhtar ; Khim, Nimol ; Lim, Pharath ; Volney, Béatrice ; Ekala, Marie Thérèse ; Bouchier, Christiane ; Esterre, Philippe ; Fandeur, Thierry ; Mercereau-Puijalon, Odile</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-hal_primary_oai_HAL_pasteur_00590989v13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antimalarials</topic><topic>Artemisinins</topic><topic>Drug Resistance, Multiple</topic><topic>Genotype</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Parasitic Sensitivity Tests</topic><topic>Parasitology</topic><topic>Plasmodium falciparum</topic><topic>Point Mutation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jambou, Ronan</creatorcontrib><creatorcontrib>Legrand, Eric</creatorcontrib><creatorcontrib>Niang, Makhtar</creatorcontrib><creatorcontrib>Khim, Nimol</creatorcontrib><creatorcontrib>Lim, Pharath</creatorcontrib><creatorcontrib>Volney, Béatrice</creatorcontrib><creatorcontrib>Ekala, Marie Thérèse</creatorcontrib><creatorcontrib>Bouchier, Christiane</creatorcontrib><creatorcontrib>Esterre, Philippe</creatorcontrib><creatorcontrib>Fandeur, Thierry</creatorcontrib><creatorcontrib>Mercereau-Puijalon, Odile</creatorcontrib><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Lancet (British edition)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jambou, Ronan</au><au>Legrand, Eric</au><au>Niang, Makhtar</au><au>Khim, Nimol</au><au>Lim, Pharath</au><au>Volney, Béatrice</au><au>Ekala, Marie Thérèse</au><au>Bouchier, Christiane</au><au>Esterre, Philippe</au><au>Fandeur, Thierry</au><au>Mercereau-Puijalon, Odile</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6</atitle><jtitle>The Lancet (British edition)</jtitle><date>2005-12-03</date><risdate>2005</risdate><volume>366</volume><issue>9501</issue><spage>1960</spage><epage>3</epage><pages>1960-3</pages><issn>0140-6736</issn><eissn>1474-547X</eissn><abstract>Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (&gt;30 nmol/L) artemether IC50s (p&lt;0.0001, Mann-Whitney). All resistant isolates came from areas with uncontrolled use of artemisinin derivatives. This rise in resistance indicates the need for increased vigilance and a coordinated and rapid deployment of drug combinations.</abstract><pub>Elsevier</pub><pmid>16325698</pmid><doi>10.1016/S0140-6736(05)67787-2</doi><orcidid>https://orcid.org/0000-0003-3549-1543</orcidid><orcidid>https://orcid.org/0000-0003-3549-1543</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0140-6736
ispartof The Lancet (British edition), 2005-12, Vol.366 (9501), p.1960-3
issn 0140-6736
1474-547X
language eng
recordid cdi_hal_primary_oai_HAL_pasteur_00590989v1
source Business Source Ultimate; Elsevier
subjects Animals
Antimalarials
Artemisinins
Drug Resistance, Multiple
Genotype
Humans
Life Sciences
Microbiology and Parasitology
Parasitic Sensitivity Tests
Parasitology
Plasmodium falciparum
Point Mutation
title Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T05%3A36%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-hal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Resistance%20of%20Plasmodium%20falciparum%20field%20isolates%20to%20in-vitro%20artemether%20and%20point%20mutations%20of%20the%20SERCA-type%20PfATPase6&rft.jtitle=The%20Lancet%20(British%20edition)&rft.au=Jambou,%20Ronan&rft.date=2005-12-03&rft.volume=366&rft.issue=9501&rft.spage=1960&rft.epage=3&rft.pages=1960-3&rft.issn=0140-6736&rft.eissn=1474-547X&rft_id=info:doi/10.1016/S0140-6736(05)67787-2&rft_dat=%3Chal%3Eoai_HAL_pasteur_00590989v1%3C/hal%3E%3Cgrp_id%3Ecdi_FETCH-hal_primary_oai_HAL_pasteur_00590989v13%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/16325698&rfr_iscdi=true