Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2008-10, Vol.26 (2), p.116-122
• Main Authors: Sahambi, Sukhdeep K., Visser, Jenny A., Themmen, Axel P.N., Mayer, Loretta P., Devine, Patrick J.
• Format: Article
• Language: English
• Subjects: 4-Vinylcyclohexene diepoxide; Animals; Anti-Mullerian Hormone; Anti-Mullerian Hormone - blood; Anti-Mullerian Hormone - metabolism; Anti-Müllerian hormone; Biological and medical sciences; Biological Markers; Biomarker; Biomarkers - blood; Biomarkers - metabolism; Body Weight; Body Weight - drug effects; Cyclohexenes; Cyclohexenes - toxicity; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Human health and pathology; Immunohistochemistry; Infectious diseases; Life Sciences; Medical sciences; Menopause; Mice; Mice, Inbred C57BL; Ovarian Follicle; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; Ovarian Follicle - pathology; Teratology. Teratogens; Time Factors; Toxicology; Vinyl Compounds; Vinyl Compounds - toxicity
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2008.07.005

A chemically induced model of ovarian failure has been developed in rodents, and was used to test whether or not anti-Müllerian hormone (AMH) can be used as a non-invasive measure of primordial follicle numbers. Repeated exposures of mice to 4-vinylcyclohexene diepoxide (VCD) induce loss of primordial and earliest growing ovarian follicles. An accelerated exposure regimen was used to eliminate small ovarian follicles in C57BL6/J mice (240 mg VCD/kg/day, 5 days, i.p.). Follicle populations were determined and correlated with circulating AMH levels. Exposures decreased only primordial and small primary follicles by 96% on day 16 after initiating exposures, followed by almost complete follicle elimination on days 37–100. AMH levels in VCD-exposed mice were similar to vehicle-treated mice on day 16, but became significantly lower or undetectable at later time points. Thus, AMH correlated well with growing follicle numbers. AMH only correlated with primordial follicles at time points after ovarian insult at which their loss led to decreased growing follicle numbers.