Release of the cytosolic tryparedoxin peroxidase into the incubation medium and a different profile of cytosolic and mitochondrial peroxiredoxin expression in H2O2-treated Trypanosoma cruzi tissue culture-derived trypomastigotes

• Published in: Experimental parasitology 2013-03, Vol.133 (3), p.287-293
• Main Authors: Gadelha, F.R., Gonçalves, C.C., Mattos, E.C., Alves, M.J.M., Piñeyro, M.D., Robello, C., Peloso, E.F.
• Format: Article
• Language: English
• Subjects: antioxidants; Blotting, Western; Cell Line; Cytosol; Cytosol - enzymology; Hydrogen Peroxide; Hydrogen Peroxide - pharmacology; Life Sciences; Mitochondria; Mitochondria - enzymology; Oxidative Stress; parasites; parasitology; Peroxidases; Peroxidases - metabolism; peroxiredoxin; Peroxiredoxins; Peroxiredoxins - metabolism; Protozoan Proteins; Protozoan Proteins - metabolism; Trypanosoma cruzi; Trypanosoma cruzi - drug effects; Trypanosoma cruzi - enzymology; Tryparedoxin peroxidases; trypomastigotes
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2012.12.007

TcCPx expression analysis in tissue-culture derived trypomastigotes and in the supernatant of the H2O2-incubated medium. [Display omitted] ► Release of cytosolic tryparedoxin peroxidase into the H2O2-incubation medium. ► Mitochondrial tryparedoxin peroxidase expression increased with 10μM H2O2. ► Different peroxiredoxins expression in trypomastigotes upon H2O2-treatment. ► Trypomastigotes integrity was not altered upon H2O2 treatment. ► Trypomastigotes have higher peroxiredoxins expression levels than epimastigotes. Trypanosoma cruzi antioxidant enzymes are among the factors that guarantee parasite survival and maintain infection, enabling T. cruzi to cope with oxidative stress. Herein, the expression of cytosolic (TcCPx) and mitochondrial (TcMPx) tryparedoxin peroxidases was evaluated in tissue culture-derived trypomastigotes upon incubation with different concentrations of H2O2. TcCPx expression slightly increased (5.4%) in cells submitted to 10μM H2O2 treatment when compared to the control, but decreased when higher H2O2 concentrations (20–50μM) were employed. Under these conditions, TcMPx expression increased (∼53%) with 10μM-treatment compared to the control, followed by a reduction that reached ∼46% of the control when using the highest concentration tested. Interestingly, in the supernatant of the incubations, TcCPx, but not TcMPx, was detected, and its levels increased concomitantly with its decreased expression in the intracellular compartment. Our data show that peroxiredoxins in the tissue culture-derived trypomastigote can be modulated under oxidative stress and are present in higher amounts when compared to the epimastigote stage of T. cruzi. Additionally, due to the different expression patterns observed upon H2O2-treatment, each peroxiredoxin may play a distinct role in protecting the parasite under oxidative stress conditions.