Ini1/hSNF5 is dispensable for retrovirus-induced cytoplasmic accumulation of PML and does not interfere with integration

• Published in: FEBS letters 2004-12, Vol.578 (3), p.291-296
• Main Authors: Boese, Annette, Sommer, Peter, Gaussin, Armelle, Reimann, Andreas, Nehrbass, Ulf
• Format: Article
• Language: English
• Subjects: Annexin A5; Annexin A5 - metabolism; Antibiotics, Antineoplastic; Antibiotics, Antineoplastic - pharmacology; Blotting, Western; Cell Fractionation; Chromosomal Proteins, Non-Histone; Cytoplasm; Cytoplasm - metabolism; DNA-Binding Proteins; DNA-Binding Proteins - drug effects; DNA-Binding Proteins - metabolism; double-stranded RNA; dsRNA; EGFP; enhanced green fluorescence protein; FACS; Fatty Acids, Unsaturated; Fatty Acids, Unsaturated - pharmacology; fluorescence activated cell sorting; Fluorescent Antibody Technique; HeLa Cells; HIV; Human immunodeficiency virus; Humans; Ini1; integrase interactor 1; Integrase interactor 1/hSNF5; leptomycin B; Life Sciences; LMB; Microbiology and Parasitology; Neoplasm Proteins; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - drug effects; Nuclear Proteins; Nuclear Proteins - biosynthesis; Nuclear Proteins - drug effects; PBS; phosphate-buffered saline; PIC; PML; PML oncogenic domain; POD; Polymerase Chain Reaction; preintegration complex; Promyelocytic Leukemia Protein; propidium iodide; Retroviral integration; Retroviridae Infections; Retroviridae Infections - enzymology; Retroviridae Infections - metabolism; RNA Interference; RNAi; siRNA; small inhibitory RNA; SMARCB1 Protein; Transcription Factors; Transcription Factors - biosynthesis; Transcription Factors - drug effects; Tumor Suppressor Proteins; vesicular stomatitis virus glycoprotein; Virology; Virus Integration; Virus Integration - physiology; VSV-G; wild type
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2004.11.016

Retroviral infection triggers the cytoplasmic translocation of two Crm1-dependent shuttle factors, namely the Ini1 (integrase interactor 1, hSNF5) and the promyelocytic leukemia (PML) protein. Blocking nuclear export of shuttle factors by leptomycin B increases the efficiency of retroviral integration, suggesting that some may mediate antiviral activity. While PML was shown to counteract proviral establishment, it remained unclear whether Ini1, a protein implicated in various processes during human immunodeficiency virus replication, has the same potential. Employing RNA interference-mediated knock-down of Ini1, we show here that the simultaneous accumulation of both proteins in the cytoplasm likely reflects two non-interdependent phenomena. Furthermore, Ini1 does not interfere with retroviral integration, as cells lacking Ini1 show no increased infection susceptibility.