Culture of Borrelia persica and its flagellar antigen in vitro

Borrelia persica is a strain seen only in the Middle East and responsible for relapsing fever. These spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. Diagnosis of the disease is a problem and requires...

Full description

Saved in:
Bibliographic Details
Published in:Pakistan journal of biological sciences 2014-01, Vol.17 (2), p.190-197
Main Authors: Zamani, Zahra, Arjmand, Mohammad, Oreiz, Farzad, Soleimani, Mohammad, Hosseini, Seyed Hossein, Assmar, Mehdi, Javadian, Saifeddin, Saghiri, Reza, Pourfallah, Fatemeh
Format: Article
Language:English
Subjects:
Antigens, Bacterial
Antigens, Bacterial - immunology
Borrelia
Borrelia - growth & development
Borrelia - immunology
Cross Reactions
Flagella
Flagella - immunology
Life Sciences
Microbiology and Parasitology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Borrelia persica is a strain seen only in the Middle East and responsible for relapsing fever. These spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. Diagnosis of the disease is a problem and requires a fixed antigen like the flagellar antigen. In vitro culture of B. persica was carried out for the first time and flagellar antigen was purified from culture. 10% SDS was added to the mixture to dissolve the cell wall and then the solution was sheared in an Omni mixer. Electron microscopy confirmed the purity of a 42 KDa periplasmic antigen as revealed by SDS-PAGE. Indirect haemagglutination kits were designed using the pure flagella and tested for cross reactivity with another relapsing fever spirochaete Borrelia microtii positive serum. The kit showed 98% sensitivity and 95% specificity.
ISSN:1028-8880
1812-5735
DOI:10.3923/pjbs.2014.190.197