Are Variable-Number Tandem Repeats Appropriate for Genotyping Mycobacterium leprae?

Comparative genomics analysis of the Tamil Nadu strain of Mycobacterium leprae has uncovered several polymorphic sites with potential as epidemiological tools. In this study we compared the stability of two different markers of genomic biodiversity of M. leprae in several biopsy samples isolated fro...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2008-07, Vol.46 (7), p.2291-2297
Main Authors: Monot, Marc, Honoré, Nadine, Balière, Charlotte, Ji, Baohong, Sow, Samba, Brennan, Patrick J, Cole, Stewart T
Format: Article
Language:English
Subjects:
Adult
Aged
Alleles
Bacterial Typing Techniques
Bacterial Typing Techniques - methods
Bacteriology
Biochemistry, Molecular Biology
Biological and medical sciences
DNA Primers
DNA Primers - genetics
DNA, Bacterial
DNA, Bacterial - genetics
Female
Fundamental and applied biological sciences. Psychology
Humans
Leprosy
Leprosy - microbiology
Life Sciences
Male
Mali
Microbiology
Middle Aged
Minisatellite Repeats
Miscellaneous
Molecular biology
Molecular Epidemiology
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium leprae
Mycobacterium leprae - classification
Mycobacterium leprae - genetics
Mycobacterium leprae - isolation & purification
Polymorphism, Genetic
Polymorphism, Single Nucleotide
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Summary:Comparative genomics analysis of the Tamil Nadu strain of Mycobacterium leprae has uncovered several polymorphic sites with potential as epidemiological tools. In this study we compared the stability of two different markers of genomic biodiversity of M. leprae in several biopsy samples isolated from the same leprosy patient. The first type comprises five different variable-number tandem repeats (VNTR), while the second is composed of three single nucleotide polymorphisms (SNP). Contrasting results were obtained, since no variation was seen in the SNP profiles of M. leprae from 42 patients from 7 different locations in Mali whereas the VNTR profiles varied considerably. Furthermore, since variation in the VNTR pattern was seen not only between different isolates of M. leprae but also between biopsy samples from the same patient, these VNTR may be too dynamic for use as epidemiological markers for leprosy.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00239-08