Molecular characterization and antibiotic resistance of Salmonellain children with acute gastroenteritis in Abuja, Nigeria

In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including Salmonella, remains a powerful tool for effective management, surveillance, and control.Diarr...

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Bibliographic Details
Published in:Journal of infection in developing countries 2014-06, Vol.8 (6), p.712-9
Main Authors: Ifeanyi, Casmir Ifeanyichukwu Cajetan, Bassey, Bassey Enya, Ikeneche, Nkiruka Florence, Al-Gallas, Nazek
Format: Article
Language:English
Subjects:
Child, Preschool
Developing Countries
DNA, Bacterial
Drug Resistance, Bacterial
Female
Gastroenteritis
Human health and pathology
Humans
Hépatology and Gastroenterology
Infant
Life Sciences
Nigeria
Salmonella enterica
Salmonella enteritidis
Salmonella Infections
Serogroup
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Summary:In Nigeria, acute gastroenteritis in children under five years of age is a major cause of mortality and morbidity; identification and characterization of microbial agents of acute gastroenteritis, including Salmonella, remains a powerful tool for effective management, surveillance, and control.Diarrheal stool samples were directly plated onto differential and selective media to isolate Salmonella. Extended-spectrum beta-lactamases were screened using the double disk diffusion technique and by PCR targeting the blaTEM and blaCTX-M genes. Pulsed-field gel electrophoresis (PFGE) was performed usingthe PulseNet Canada Laboratory protocol for molecular subtyping using the restriction enzymes XbaI and BlnI.The serotypes identified were Salmonella enterica serovar Zanzibar (n = 5), Salmonella Brancaster (n = 3), and one isolate of Salmonella Enteritidis (phage type 1). The following levels of resistance were found among the Salmonella strains: amoxicillin, five strains (55.6%); amoxicillin-clavulanic acid, two strains (22.2%); cephalexin, five strains (55.6%); and cefuroxime, five strains (55.6%). Intermediate resistance was found in five strains (55.6%) only to amoxicillin-clavulanic acid. All isolates were susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone, and no ESBL-producing Salmonella were detected.Our findings demonstrated the involvement of three Salmonella serovars in acute gastroenteritis; resistance to penicillins and cephalosporins was common.
ISSN:2036-6590
1972-2680
DOI:10.3855/jidc.4185