Influence of very short patch mismatch repair on SOS inducing lesions after aminoglycoside treatment in Escherichia coli

Low concentrations of aminoglycosides induce the SOS response in Vibrio cholerae but not in Escherichia coli. In order to determine whether a specific factor present in E. coli prevents this induction, we developed a genetic screen where only SOS inducing mutants are viable. We identified the vsr ge...

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Bibliographic Details
Published in:Research in microbiology 2014-07, Vol.165 (6), p.476-480
Main Authors: Baharoglu, Zeynep, Mazel, Didier
Format: Article
Language:English
Subjects:
Aminoglycosides
Aminoglycosides - pharmacology
Anti-Bacterial Agents
Anti-Bacterial Agents - pharmacology
Antibiotics
Bacteriology
Biochemistry, Molecular Biology
DNA Mismatch Repair
DNA repair
DNA Repair Enzymes
DNA Repair Enzymes - genetics
DNA Repair Enzymes - metabolism
Endodeoxyribonucleases
Endodeoxyribonucleases - genetics
Endodeoxyribonucleases - metabolism
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - physiology
Escherichia coli Proteins
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Genetics
Life Sciences
Microbiology and Parasitology
Molecular biology
SOS
SOS Response (Genetics)
Sub-MIC
Vibrio cholerae
VSPR
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Summary:Low concentrations of aminoglycosides induce the SOS response in Vibrio cholerae but not in Escherichia coli. In order to determine whether a specific factor present in E. coli prevents this induction, we developed a genetic screen where only SOS inducing mutants are viable. We identified the vsr gene coding for the Vsr protein of the very short patch mismatch repair (VSPR) pathway. The effect of mismatch repair (MMR) mutants was also studied. We propose that lesions formed upon aminoglycoside treatment are preferentially repaired by VSPR without SOS induction in E. coli and by MMR when VSPR is impaired.
ISSN:0923-2508
1769-7123
0923-2508
DOI:10.1016/j.resmic.2014.05.039