A model to study the effects of a viral inactivator (β-propiolactone) on DNA ligation and gene expression in E. coli and Cos cells

• Published in: Vaccine 1999, Vol.17 (1), p.95-98
• Main Authors: Fathallah, Dahmani M, Zerria, Khaled, Barbouche, M.Ridha, Dellagi, Koussay
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents, Local - pharmacology; Applied microbiology; Biochemistry, Molecular Biology; Biological and medical sciences; COS Cells - drug effects; COS Cells - metabolism; COS Cells - physiology; DNA; DNA - drug effects; DNA - genetics; DNA - metabolism; DNA Ligases - genetics; DNA Ligases - metabolism; DNA, Bacterial - drug effects; DNA, Bacterial - metabolism; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression - drug effects; Immunology; Life Sciences; ligation; Microbiology; Models, Biological; Propiolactone - pharmacology; Transfection; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccinology; β-propiolactone
• ISSN: 0264-410X; 1873-2518; 0264-410X
• DOI: 10.1016/S0264-410X(98)00087-5

An experimental model to study the effects of viral inactivators on the biological properties of DNA was developed. β-propiolactone (βPL) was used in this model and its effects on ligation, transfer and gene expression of naked DNA were assessed. Evidence that βPL impairs these two major DNA functions are presented. The amounts of βPL that alter or abolish gene expression and prevent DNA cohesive ends ligation were determined. Based on these observations, it was concluded that this experimental approach could be used to study the effects on the biological properties of DNA of other inactivators used in vaccine preparations.