The L17 ribosomal protein of Bacillus subtilis binds preferentially to curved DNA

We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 resp...

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Bibliographic Details
Published in:Biochimie 2000, Vol.82 (1), p.85-91
Main Authors: Zouine, M., Beloin, C., Ghelis, C., Le Hégarat, F.
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
Bacterial Proteins
Cell Division - genetics
curved DNA
DNA, Bacterial - chemistry
DNA, Bacterial - metabolism
DNA-Binding Proteins - isolation & purification
DNA-Binding Proteins - metabolism
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
HBsu
L17
Life Sciences
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Ribosomal Proteins - metabolism
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Summary:We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.
ISSN:0300-9084
1638-6183
DOI:10.1016/S0300-9084(00)00184-X