APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome

Abstract Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA...

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Published in:The Journal of infectious diseases 2022-09, Vol.226 (5), p.891-895
Main Authors: Khalfi, Pierre, Suspène, Rodolphe, Caval, Vincent, Thiers, Valérie, Beauclair, Guillaume, Marchio, Agnès, Bekondi, Claudine, Amougou Atsama, Marie, Camengo-Police, Serge Magloire, Noah Noah, Dominique, Njouom, Richard, Blanc, Hervé, Vallet, Thomas, Vignuzzi, Marco, Pineau, Pascal, Vartanian, Jean Pierre
Format: Article
Language:English
Subjects:
Dimerization
Genomes
Hepatitis
Hepatitis B
HIV
Human immunodeficiency virus
Isoleucine
Life Sciences
Microbiology and Parasitology
Next-generation sequencing
Single-nucleotide polymorphism
Virology
Viruses
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Summary:Abstract Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I. Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA. In this study, we demonstrated in culture and in patients that HBV could be edited by APOBEC3CS188I. This enzyme led to an enhanced editing activity in a more specific 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of APOBEC3CS188I.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/jiac003