cpbAg1 encodes an active carboxypeptidase B expressed in the midgut of Anopheles gambiae

We previously used differential display to identify several Anopheles gambiae genes, whose expression in the mosquito midgut was regulated upon ingestion of Plasmodium falciparum. Here, we report the characterization of one of these genes, cpbAg1, which codes for the first zinc‐carboxypeptidase B id...

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Bibliographic Details
Published in:Insect molecular biology 2005-04, Vol.14 (2), p.163-174
Main Authors: Lavazec, C, Bonnet, S, Thiery, I, Boisson, B, Bourgouin, C
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Anopheles - genetics
Anopheles - metabolism
Anopheles gambiae
Baculovirus
Base Sequence
Blotting, Western
carboxypeptidase B
Carboxypeptidase B - genetics
Carboxypeptidase B - metabolism
carboxypeptidases
Cluster Analysis
complementary DNA
Conserved Sequence - genetics
Culicidae
digestive enzyme
digestive enzymes
DNA Primers
Down-Regulation - physiology
Gastrointestinal Tract - metabolism
gene expression regulation
gene family
Genes, Insect - genetics
hematophagy
Life Sciences
messenger RNA
midgut
Molecular Sequence Data
multigene family
Multigene Family - genetics
nucleotide sequences
Phylogeny
Plasmodium falciparum
Protein Conformation
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sequence Alignment
Sequence Analysis, DNA
zinc-carboxypeptidases
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Summary:We previously used differential display to identify several Anopheles gambiae genes, whose expression in the mosquito midgut was regulated upon ingestion of Plasmodium falciparum. Here, we report the characterization of one of these genes, cpbAg1, which codes for the first zinc‐carboxypeptidase B identified in An. gambiae and in any insect. Expression of cpbAg1 in baculovirus gave rise to an active enzyme, and determination of the N‐terminal amino acids confirmed that CPBAg1 contains a signal peptide and a pro‐peptide, typical features of digestive zinc carboxypeptidases. cpbAg1 mRNA was mainly produced in the mosquito midgut, where it accumulated in unfed females and was rapidly down‐regulated upon blood feeding. Annotation of the An. gambiae genome predicts twenty‐three sequences coding for zinc‐carboxypeptidases of which only two (cpbAg1 and cpbAg2) are expressed at a significant level in the mosquito midgut.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.2004.00541.x