Degradation of 1,2-dichlorobenzene by a Pseudomonas sp

A pseudomonas sp. that was capable of growth on 1,2-dichlorobenzene (o-DCB) or chlorobenzene as a sole source of carbon and energy was isolated by selective enrichment from activated sludge. The initial steps involved in the degradation of o-DCB were investigated by isolation of metabolites, respiro...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1988-02, Vol.54 (2), p.294-301
Main Authors: Haigler, B.E, Nishino, S.F, Spain, J.C
Format: Article
Language:English
Subjects:
activated sludge
AISLAMIENTO (TECNICA)
Alcohol Oxidoreductases - metabolism
Bacteriology
BENCENO
BENZENE
biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biological treatment of waters
Biotechnology
Catechol 1,2-Dioxygenase
Catechols - metabolism
Chemical Phenomena
Chemistry
Chlorides - metabolism
Chlorobenzenes - metabolism
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
DEGRADATION
DETERIORO
Dioxygenases
Environment and pollution
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry
Industrial applications and implications. Economical aspects
ISOLEMENT
Maleates - metabolism
Metabolism. Enzymes
Microbiology
o-dichlorobenzene
Oxidation-Reduction
Oxidoreductases
Oxidoreductases Acting on CH-CH Group Donors
Oxygenases - metabolism
PESTICIDE
PLAGUICIDAS
PSEUDOMONAS
Pseudomonas - enzymology
Pseudomonas - growth & development
Pseudomonas - metabolism
Sewage
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Summary:A pseudomonas sp. that was capable of growth on 1,2-dichlorobenzene (o-DCB) or chlorobenzene as a sole source of carbon and energy was isolated by selective enrichment from activated sludge. The initial steps involved in the degradation of o-DCB were investigated by isolation of metabolites, respirometry, and assay of enzymes in cell extracts. Extracts of o-DCB-grown cells converted radiolabeled o-DCB to 3,4-dichloro-cis-1,2-dihydroxycyclohexa-3,5-diene (o-DBC dihydrodiol). 3,4-Dichlorocatechol and o-DCB dihydrodiol accumulated in culture fluids of cells exposed to o-DCB. The results suggest that o-DCB is initially converted by a dioxygenase to dihydrodiol, which is converted to 3,4-dichlorocatechol by a NAD+ -dependentdehydrogenase. Ring cleavage of 3,4-dichlorocatechol is by a catechol 1,2-oxygenase to form 2,3-dichloro-cis,cis-muconate. Preliminary results indicate that chloride is eliminated during subsequent lactonization of the 2,3-dichloro-cis,cis-muconate, followed by hydrolysis to form 5-chloromaleylacetic acid
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.54.2.294-301.1988