Characterization of chloroethylene dehalogenation by cell extracts of Desulfomonile tiedjei and its relationship to chlorobenzoate dehalogenation

We characterized the reductive dehalogenation of tetrachloroethylene in cell extracts of Desulfomonile tiedjei and compared it with this organism's 3-chlorobenzoate dehalogenation activity. Tetrachloroethylene was sequentially dehalogenated to trichloro- and dichloroethylene; there was no evide...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1996-08, Vol.62 (8), p.2850-2853
Main Authors: Townsend, G.T. (University of Oklahoma, Norman, OK.), Suflita, J.M
Format: Article
Language:English
Subjects:
ACIDE BENZOIQUE
ACIDO BENZOICO
AGUAS SUBTERRANEAS
ANAEROBIOSE
ANAEROBIOSIS
BIODECONTAMINACION
BIODEGRADACION
BIODEGRADATION
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
BIOREMEDIATION
Biotechnology
CHLORE
CLORO
COMPOSE ORGANOCHLORE
COMPUESTO ORGANICO DEL CLORO
Contamination
Desulfomonile tiedjei
EAU SOUTERRAINE
Fundamental and applied biological sciences. Psychology
Groundwater
HIDROCARBUROS HALOGENADOS
HYDROCARBURE HALOGENE
INSECTICIDAS
INSECTICIDE
LIASAS
LYASE
Microbiology
MICROORGANISME
MICROORGANISMOS
Microorganisms
Mission oriented research
Physiology and metabolism
POLLUTION DE L'EAU
POLUCION DEL AGUA
REACCIONES QUIMICAS
REACTION CHIMIQUE
REDUCCION
REDUCTION
SOLVANT
SOLVENTES
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Summary:We characterized the reductive dehalogenation of tetrachloroethylene in cell extracts of Desulfomonile tiedjei and compared it with this organism's 3-chlorobenzoate dehalogenation activity. Tetrachloroethylene was sequentially dehalogenated to trichloro- and dichloroethylene; there was no evidence for dichloroethylene dehalogenation. Like the previously characterized 3-chlorobenzoate dehalogenation activity, tetrachloroethylene dehalogenation was heat sensitive, not oxygen labile, and increased in proportion to the amount of protein in assay mixtures. In addition, both dehalogenation activities were dependent on hydrogen or formate as an electron donor and had an absolute requirement for either methyl viologen or triquat as an electron carrier in vitro. Both activities appear to be catalyzed by integral membrane proteins with similar solubilization characteristics. Dehalogenation of tetrachloroethylene was inhibited by 3-chlorobenzoate but not by the structural isomers 2- and 4-chlorobenzoate. The last two compounds are not substrates for D. tiedjei. These findings lead us to suggest that the dehalogenation of tetrachloroethylene in D. tiedjei is catalyzed by a dehalogenase previously thought to be specific for meta-halobenzoates
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.62.8.2850-2853.1996