Degradation of Fluorobenzene by Rhizobiales Strain F11 via ortho Cleavage of 4-Fluorocatechol and Catechol

The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluoroc...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2006-11, Vol.72 (11), p.7413-7417
Main Authors: Carvalho, Maria F, Ferreira, Maria Isabel M, Moreira, Irina S, Castro, Paula M.L, Janssen, Dick B
Format: Article
Language:English
Subjects:
Alphaproteobacteria - enzymology
Alphaproteobacteria - growth & development
Alphaproteobacteria - metabolism
Bacteria
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Catechols - chemistry
Catechols - metabolism
Chromatography
Culture Media
Fluorobenzenes - metabolism
Fundamental and applied biological sciences. Psychology
Metabolism
Microbiology
Oxygen Consumption
Studies
Substrate Specificity
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Summary:The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.01162-06