Heterologous Expression of BetL, a Betaine Uptake System, Enhances the Stress Tolerance of Lactobacillus salivarius UCC118

Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial be...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2006-03, Vol.72 (3), p.2170-2177
Main Authors: Sheehan, Vivien M, Sleator, Roy D, Fitzgerald, Gerald F, Hill, Colin
Format: Article
Language:English
Subjects:
abiotic stress
Anti-Bacterial Agents - pharmacology
bacterial proteins
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
betaine
Betaine - metabolism
Biological and medical sciences
Biotechnology
Carrier Proteins - genetics
Carrier Proteins - metabolism
cold stress
Colony Count, Microbial
cooling
freeze drying
Freezing
Fundamental and applied biological sciences. Psychology
gene expression
gene induction
genetically engineered microorganisms
Heat-Shock Response
Lactobacillus - genetics
Lactobacillus - growth & development
Lactobacillus - metabolism
Lactobacillus - physiology
Lactobacillus salivarius
Listeria monocytogenes
Listeria monocytogenes - genetics
Listeria monocytogenes - metabolism
Microbiology
nisin
Nisin - pharmacology
nisin-controlled gene expression
Osmolar Concentration
osmoregulation
osmotic pressure
physiological transport
Physiology and Biotechnology
pressure treatment
probiotics
spray drying
stress tolerance
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Summary:Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.72.3.2170-2177.2006